Method for increasing slowly digestible starch content in starch
A technology of slow-digesting starch and starch, which is applied in agricultural product processing and biological fields, can solve the problems of toxic substance residue, high cost, unsuitable for industrial production, etc., achieve broad market prospects, low cost, and increase the content of slow-digesting starch.
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Embodiment 1
[0018] Dissolve cornstarch in water to obtain 1% starch milk, heat it with magnetic stirring for 30 min, adjust its pH to 7.0 after cooling, and add 10 U / g (based on starch dry basis) α-amylase to react for 20 min. After the reaction, inactivate the enzyme in a boiling water bath for 20 minutes, centrifuge at 6,000 r / min for 5 minutes, wash the supernatant with deionized water, collect the solids and refrigerate at 4°C for 5 days, then dry them in an oven at 45°C for 12 hours, and grind them for 100 Mesh sieve to obtain modified starch. The control group is the sample obtained by adding inactivated α-amylase. The content of slow digestible starch in starch was determined by Englyst in vitro simulation method. Compared with the control group, the content of slow digestible starch in modified corn starch increased by 30%.
Embodiment 2
[0020] Dissolve wheat starch in water to obtain 3% starch milk, heat it with magnetic stirring for 30 min, adjust its pH to 8.0 after cooling, and add 15 U / g (based on starch dry basis) α-amylase to react for 10 min. After the reaction, inactivate the enzyme in a boiling water bath for 20 min, centrifuge at 6,000 r / min for 5 min, wash the supernatant with deionized water, collect the solid matter and refrigerate at 4°C for 7 days, dry it in an oven at 45°C for 12 h, and grind for 100 Mesh sieve to obtain modified starch. The control group is the sample obtained by adding inactivated α-amylase. The content of slow digestible starch in starch was determined by Englyst in vitro simulation method. Compared with the control group, the content of slow digestible starch in modified wheat starch increased by 10%.
Embodiment 3
[0022] Dissolve sweet potato starch in water to obtain 5% starch milk, heat it with magnetic stirring for 30 min, adjust its pH to 6.0 after cooling, and add 5 U / g (based on starch dry basis) α-amylase to react for 30 min. After the reaction, inactivate the enzyme in a boiling water bath for 20 min, centrifuge at 6000 r / min for 5 min, wash the supernatant with deionized water, collect the solid matter and refrigerate at 4°C for 9 days, dry in an oven at 45°C for 12 hours, and grind for 100 Mesh sieve to obtain modified starch. The control group is the sample obtained by adding inactivated α-amylase. The content of slow digestible starch in starch was determined by Englyst in vitro simulation method. Compared with the control group, the content of slow digestible starch in modified sweet potato starch increased by 20%.
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