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Antibacterial composite microbial preparation under low pH condition

A technology of compound microorganisms and preparations, applied in the field of microorganisms, can solve problems such as poor enteritis effects, achieve good development and application prospects, be suitable for industrial production, and have diverse modes of action

Pending Publication Date: 2017-03-22
天益健康科学研究院(镇江)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Existing Probiotics Are Ineffective in Treating Enteritis

Method used

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  • Antibacterial composite microbial preparation under low pH condition
  • Antibacterial composite microbial preparation under low pH condition
  • Antibacterial composite microbial preparation under low pH condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Isolation of Lactobacillus reuteri TR02

[0046] 1. Strains, medium and culture conditions

[0047] (1) Bacterial source

[0048] Feces of healthy soldiers from a military region in China.

[0049] (2) culture medium

[0050] Plate medium: 1000mL distilled water, 10g peptone, 20g glucose, 9g yeast powder, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 2g triammonium citrate, 0.5g cysteine, 0.2g magnesium sulfate, 0.05g manganese sulfate, Tween 1ml, agar powder 15-20g, pH5.5-6.5.

[0051] 2. Isolation and identification of strains

[0052] (1) Isolation of strains

[0053] Take an appropriate amount of fresh feces from the pre-sterilized glove box and place them in a sterile test tube filled with sterile medium, vortex and mix well;

[0054] Take the feces solution that was fully shaken and vortexed as the stock solution, and make a ten-fold gradient dilution to a suitable gradient, take 100 μl and evenly spread it on the MRS+0.05% cysteine ​​solid plate mediu...

Embodiment 2

[0121] Isolation of Lactobacillus rhamnosus TR08

[0122] 1. Strains, medium and culture conditions

[0123] (1) Bacterial source

[0124] Feces of healthy soldiers from a military region in China

[0125] (2) culture medium

[0126] Plate medium: 1000mL distilled water, 10g peptone, 20g glucose, 9g yeast powder, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 2g triammonium citrate, 0.5g cysteine, 0.2g magnesium sulfate, 0.05g manganese sulfate, Tween 1ml, agar powder 15-20g, pH5.5-6.5.

[0127] 2. Isolation and identification of strains

[0128] (1) Isolation of strains

[0129] Take an appropriate amount of fresh feces from the pre-sterilized glove box and place them in a sterile test tube filled with sterile medium, vortex and mix well;

[0130] Take the feces solution that was fully shaken and vortexed as the stock solution, and make a ten-fold gradient dilution to a suitable gradient, take 100 μl and evenly spread it on the MRS+0.05% cysteine ​​solid plate me...

Embodiment 3

[0196] Embodiment 3 antibacterial efficacy

[0197] Lactobacillus rhamnosus TR08 and Lactobacillus reuteri TR02 are mixed with Candida albicans culture fluid, and the mass ratio of Lactobacillus reuteri TR02 and Lactobacillus rhamnosus TR08 is 1.2:1, measure the OD value and pH value at 0, 6, 9, 12, and 24 hours respectively, such as Figure 17 As shown, Lactobacillus rhamnosus TR08 and Lactobacillus reuteri TR02 have obvious antibacterial effect on Candida albicans, and can significantly reduce the pH value of the environment.

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Abstract

The invention discloses an antibacterial composite microbial preparation under the low pH condition. The preparation comprises Lactobacillus reuteri TR02 and Lactobacillus rhamnosus TR08; the Lactobacillus reuteri TR02 is preserved in China Center for Type Culture Collection, the preservation number is CCTCC No.M 2016546; the Lactobacillus rhamnosus TR08 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO.M 2016548. The composite microbial preparation is simple in culture condition, short in fermentation time, easy to preserve and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a bacteriostatic compound microbial preparation under low pH conditions. Background technique [0002] Ulcerative colitis (UC), cryptitis, and Rohn's disease are major types of inflammatory bowel disease (IBD), which is characterized by chronic inflammation in the intestine. Clinical signs are diarrhea, abdominal pain, occasional rectal bleeding, weight loss, tiredness, and sometimes fever. Although it can occur at any age, IBD is most common in adolescents and young adults, so these individuals may suffer from delayed development and stunted growth. Statistics show that the annual prevalence of UC in the United States is 200 / 100,000 people, and the cost of treating UC is about 100 to 150 million US dollars. As of 2012, the total number of UC patients is 2.5 times that of 2000, and the recurrence rate is 72%; In recent years, the number of patients with UC in my country has sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P1/00A61P31/10A61P1/04C12R1/225
CPCA61K35/747C12R2001/225C12N1/205A61K2300/00
Inventor 李珮铷贾长生边高瑞
Owner 天益健康科学研究院(镇江)有限公司