Preparation method for collagen nanowire array layer cuvette

A nanowire array and collagen technology, applied in the field of improvement of cuvettes, to achieve the effect of easy operation, good hydrophilic performance, and good polarization characteristics

Inactive Publication Date: 2017-03-29
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not been reported to control the self-assembly of biomacromolecules on the crystal surface in a liquid-phase buffer solution by using the recognition function of mineral crystals and biomacromolecules in reverse.

Method used

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  • Preparation method for collagen nanowire array layer cuvette
  • Preparation method for collagen nanowire array layer cuvette
  • Preparation method for collagen nanowire array layer cuvette

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing a cuvette with a collagen nanowire array layer, the specific steps are as follows:

[0025] Step 1, make the mica sheet into a cuvette structure, stick the corner of the mica sheet with adhesive tape and tear off the upper layers to expose the smooth mica sheet;

[0026] Step 2, prepare the Na of 0.2mol / L 2 HPO 4 and 0.3mol / L NaH 2 PO 4 solution, the Na 2 HPO 4 solution with NaH 2 PO 4 The solutions were mixed to prepare a buffer solution with pH=6.8, and a 5 μg / ml collagen monomer solution was prepared with sodium phosphate buffer;

[0027] Step 3, evenly titrate the collagen solution on the mica sheet, and wash the mica sheet with buffer after 15-20 minutes;

[0028] Step 4: Titrate the buffer solution on the cleaned mica sheet, cover the mica sheet, incubate for 12 hours, wash with water, and dry naturally to obtain a cuvette with a collagen nanowire array layer.

[0029] AFM observation of the self-assembled structure of collagen on m...

Embodiment 2

[0031] A method for preparing a cuvette with a collagen nanowire array layer, the specific steps are as follows:

[0032] Step 1, make the mica sheet into a cuvette structure, stick the corner of the mica sheet with adhesive tape and tear off the upper layers to expose the smooth mica sheet;

[0033] Step 2, prepare the Na of 0.2mol / L 2 HPO 4 and 0.3mol / L NaH 2 PO 4 solution, the Na 2 HPO 4 solution with NaH 2 PO 4 The solutions were mixed to prepare a buffer solution with pH=7, and a 10 μg / ml collagen monomer solution was prepared with sodium phosphate buffer;

[0034] Step 3, evenly titrate the collagen solution on the mica sheet, and wash the mica sheet with buffer after 15-20 minutes;

[0035] Step 4: Titrate the buffer solution on the cleaned mica sheet, cover the mica sheet, incubate for 12 hours, wash with water, and dry naturally to obtain a cuvette with a collagen nanowire array layer.

[0036] AFM observation of the self-assembled structure of collagen on mi...

Embodiment 3

[0040] A method for preparing a cuvette with a collagen nanowire array layer, the specific steps are as follows:

[0041] Step 1, make the mica sheet into a cuvette structure, stick the corner of the mica sheet with adhesive tape and tear off the upper layers to expose the smooth mica sheet;

[0042] Step 2, prepare the Na of 0.2mol / L 2 HPO 4 and 0.3mol / L NaH 2 PO 4 solution, Na 2 HPO 4 solution with NaH 2 PO 4 The solutions were mixed to prepare a buffer solution with pH=8, and a 10 μg / ml collagen monomer solution was prepared with sodium phosphate buffer;

[0043] Step 3, evenly titrate the collagen solution on the mica sheet, and wash the mica sheet with buffer after 15-20 minutes;

[0044] Step 4: Titrate the buffer solution on the cleaned mica sheet, cover the mica sheet, incubate for 12 hours, wash with water, and dry naturally to obtain a cuvette with a collagen nanowire array layer.

[0045] Selected Area Diffraction (SAED) of TEM was carried out on the mica s...

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Abstract

The invention discloses a preparation method for a collagen nanowire array layer cuvette. According to the method, a phosphate buffer solution is used for preparing a collagen monomer solution, the collagen monomer solution is dropped to the surface of a micalex cuvette, covered, washed through the buffer solution and cultured for a period of time, and the collagen nanowire array layer cuvette is obtained. The cuvette has the good hydrophilic performance, and collagen on the surface has the excellent biocompatibility and can be used for cell culture. Meanwhile, two opposite mica sheets of the cuvette are nanowire arrays in the same direction, the cuvette has the very good polarization property and can be used as a mask, and in combination with an electrochemical method, the cuvette can be synthesized into a novel biosensor or prepared into a high-specificity bioprobe or synthesized into a novel micro-nanometer photoelectric material.

Description

technical field [0001] The invention relates to the improvement of a cuvette, in particular to a method for preparing a cuvette with a collagen nanowire array layer. Background technique [0002] Epitaxial growth often refers to the phenomenon that one mineral crystal grows on the surface of another mineral crystal, and the two minerals have the same crystal structure orientation on the interface. The principle of epitaxial growth is used to control and study on the surface of the crystal matrix The layer structure and growth kinetics of coating molecules have become an active field. In the past two decades, combined with molecular synthesis technology with adjustable size and function and substrates with microscopic crystal structures, under the background of ultra-high vacuum, molecular beam deposition technology is used to control the growth of organic molecular film layer crystals on the surface of substrates. The predicted new organic micro-nano optoelectronic material...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00G01N21/03
CPCC12M23/02C12M23/20G01N21/03
Inventor 曾凡喜杨德良孙铭
Owner NANJING UNIV OF SCI & TECH
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