Reagent for detection of animal influenza virus type A, detection method and application thereof

Abstract

The application discloses a reagent for detection of animal influenza virus type A, a detection method and an application thereof. The reagent includes a primer pair and a probe used for recombinase polymerase amplification of the animal influenza virus type A, wherein a forward primer of the primer pair is represented as the SEQ ID No.1, a reverse primer of the primer pair is represented as the SEQ ID No.2, and the probe is represented as the SEQ ID No.3; the 29th basic group in the probe is marked by a FAM group, the 31st basic group is replaced by dSpacer, the 34th basic group is marked by a BHQ1 group, and a 3' terminal is modified by C3Spacer. The reagent provides a novel detection scheme and approach for the detection of animal influenza virus type A. The animal influenza virus type A RPA detection method based on the reagent is quick and accurate, is low in demand on hardware equipment, is suitable for on-site quick detection and is especially suitable for departments such as animal quarantine and the like.

Description

technical field [0001] The application relates to the field of detection of type A animal influenza virus, in particular to a reagent for detection of type A animal influenza virus and its application, and a method for detecting type A animal influenza virus based on the reagent. Background technique [0002] Animal influenza virus type A is a common influenza virus belonging to the Orthomyxoviridae family. Its genome contains 8 segmented negative-strand RNAs and can be divided into 16 HA subtypes and 9 NA subtypes. The symptoms after infecting humans are mainly high fever, cough, runny nose, myalgia, etc. Most of them are accompanied by severe pneumonia. In severe cases, heart, kidney and other organs fail and cause death, with a high case fatality rate. The disease can spread through various ways such as digestive tract, respiratory tract, skin lesions and conjunctiva. Type A animal influenza viruses can infect a variety of hosts, including poultry, pigs, and humans. Amon...

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Problems solved by technology

[0003] At present, the main method for rapid detection of viral nucleic acid is the real-time fluorescent PCR method, but this method requires special thermal cyclers and other sophisticated instruments, and it is difficult to meet the detection needs under field or field conditions

At present, there is no research and report on RPA technology related to the detection of type A animal influenza

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