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Preparation method of molecular imprinting sensor for detecting melamine

A molecular imprinting and melamine technology, applied in the field of food chemical industry, can solve the problems of low detection sensitivity, difficult to meet the requirements of high cross-linking degree of polymer films, and experiments cannot be carried out quickly and effectively, and achieves enhanced sensitivity, rapid and specific selectivity. The effect of the response

Inactive Publication Date: 2017-04-12
SUZHOU UNIV OF SCI & TECH
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Problems solved by technology

However, the disadvantage of this method is that it is difficult to meet the requirement of high cross-linking degree of the polymer film, so that the experiment cannot be carried out quickly and effectively, and the detection sensitivity is low.

Method used

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  • Preparation method of molecular imprinting sensor for detecting melamine
  • Preparation method of molecular imprinting sensor for detecting melamine
  • Preparation method of molecular imprinting sensor for detecting melamine

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Embodiment Construction

[0030] In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described clearly and completely with reference to specific embodiments of the present invention and the corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0031] A method for preparing a molecularly imprinted sensor for detecting melamine, the specific preparation and analysis methods are as follows:

[0032] 1. The reagents required for the preparation of magnetic molecularly imprinted electrochemical sensors are shown in Table 1 below:

[0033] Table 1 Reagents

[0034]

[0035]

[0036] 2. The equipment requir...

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Abstract

The invention provides a preparation method of a molecular imprinting sensor for detecting melamine. The concrete preparation method comprises that a molecular imprinting sensor response to target molecule is prepared on the surface of an electrode through a chemical polymerization method by using dopamine, aniline, pyrrole or aminophenol as a functional monomer, using methyl methacrylate, trimethylolpropane-tris(3-aziridinylpropanoate) or glutaraldehyde as a cross-linking agent, using azobisisobutyronitrile or benzoyl peroxide as an initiator, using melamine as the target molecule, and using Fe3O4 magnetic nanoparticles as a signal enhancer. According to the present invention, the molecular imprinting sensor prepared through the method is used for rapidly and effectively detecting the melamine content in food, and provides rapid and specific selective response to melamine adopted as the target molecule, wherein the sensitivity of the sensor is significantly enhanced in the presence of the signal enhancer.

Description

Technical field [0001] The invention relates to the field, in particular to the field of food and chemical industry, and in particular to a method for preparing a molecularly imprinted sensor for detecting melamine. Background technique [0002] The detection methods of melamine residues in feed and food are currently limited to high performance liquid chromatography-diode array method, gas-mass spectrometry (GC-MS) method, ultra-high performance liquid chromatography-electrospray tandem mass spectrometry, reversed-phase high efficiency Liquid chromatography, high performance liquid chromatography (HPLC), high performance liquid chromatography quadrupole mass spectrometry, solid phase extraction and high performance liquid chromatography, liquid chromatography tandem mass spectrometry (LC-MS / MS), etc. The common problem of these methods is high cost and complicated pretreatment, and they have cumbersome operation, long analysis time, and not suitable for repeated processing of mu...

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Application Information

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IPC IPC(8): G01N27/26G01N27/38
CPCG01N27/26G01N27/38
Inventor 程宏英胡翠英王彦林
Owner SUZHOU UNIV OF SCI & TECH
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