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Method for separating and purifying rosmarinic acid from purple perilla

A technology for the separation and purification of rosmarinic acid, which is applied in the separation/purification of carboxylic acid esters, chemical instruments and methods, and the preparation of organic compounds, and can solve problems such as toxicity and non-compliance with green chemistry

Inactive Publication Date: 2017-04-19
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional extraction method usually combines heating with organic solvents to achieve the purpose of improving the yield. However, organic solvents not only have toxic effects and do not meet the theme of green chemistry, but also change the structure of substances through chemical reactions. , not advisable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Put 0.5kg of pulverized and sieved perilla and 7.5kg of extraction solvent acid water (pH=3.5) into a round bottom flask, and put 200g of enzyme into the round bottom flask, the extraction temperature is 80°C, and the extraction time is 45min. Extract 2 times and collect the extract; adjust the collected extract with an acidic aqueous solution (18% hydrochloric acid) to make the pH between 4 and 4.5, and then extract it with ethyl acetate, adding an amount of 2 times the volume, separate the liquids after standing still, and combine the extracts; purify the extracts with NK109 macroporous adsorption resin, collect the ethyl acetate analysis solution; concentrate the analysis solution under reduced pressure in vacuum, and the vacuum degree is -0.08~ -0.07Mpa, the concentration temperature is 30±2°C, the solvent is recovered to obtain the concentrate; then the concentrate is dried with a blower dryer at a temperature of 60°C to obtain perilla leaves with a rosmarinic acid ...

Embodiment 2

[0018] Put 0.5kg of pulverized and sieved perilla and 6.25kg of extraction solvent acid water (pH=4.5) into a round bottom flask, and put 250g of enzyme into the round bottom flask, the extraction temperature is 90°C, and the extraction time is 60min. Extract 2 times and collect the extract; adjust the collected extract with an acidic aqueous solution (18% hydrochloric acid) to make the pH between 4 and 4.5, and then extract it with ethyl acetate, adding an amount of 2 times the volume, separate the liquids after standing still, and combine the extracts; purify the extracts with NK109 macroporous adsorption resin, collect the ethyl acetate analysis solution; concentrate the analysis solution under reduced pressure in vacuum, and the vacuum degree is -0.08~ -0.07Mpa, the concentration temperature is 30±2°C, the solvent is recovered to obtain the concentrate; then the concentrate is dried with a blower dryer at a temperature of 60°C to obtain perilla leaves with a rosmarinic acid...

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PUM

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for extracting and separating rosmarinic acid from purple perilla. The method comprises the following steps of performing extraction by taking enzyme as an adjuvant in an acidic condition, then, performing extraction by utilizing ethyl acetate in the acidic condition, and performing primary purification by utilizing macro-porous adsorption resin, so that a high-purity rosmarinic acid product is obtained. The method disclosed by the invention has the advantages that: water is used as an extraction solvent in the acidic condition; compared with the traditional process that extraction is carried out by utilization of ethanol, the production cost is greatly reduced; the enzyme is added and used as the adjuvant in the extraction process; therefore, the loss of rosmarinic acid can be effectively reduced; extraction is carried out by adoption of ethyl acetate, so that the purity of the rosmarinic acid product is increased easily; and elution is carried out by utilization of ethyl acetate, so that the purity of the rosmarinic acid product is obviously increased. The rosmarinic acid prepared by the invention can be widely applied in the fields, such as health foods and cosmetics.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry and relates to a method for separating and purifying rosmarinic acid from perilla. Background technique [0002] Rosmarinic acid is a multifunctional phenolic compound and a secondary metabolic derivative in plants, which has certain physiological activities. There are multiple phenolic hydroxyl groups in the structure of rosmarinic acid, and the number has an important relationship with the antioxidant activity of rosmarinic acid. Rosmarinic acid is a water-soluble substance, light yellow powder, and it will appear blue-green when it meets ferric trichloride ferricyanide solution. The development of spectroscopic technology has provided very convenient conditions for the identification and structural analysis of natural products. With the gradual determination of the structure of rosmarinic acid, people's research on rosmarinic acid has become increasingly active, and rosmarinic acid has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C67/54C07C67/56C07C67/58C07C69/732
CPCC07C67/48C07C67/54C07C67/56C07C67/58
Inventor 姚德坤孙雷
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD