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Phycocyanin with high stability as well as preparation method, composition and application thereof

A phycocyanin and stability technology, applied in the field of natural pigment preparation, can solve the problems of fading, poor stability of cyanobacteria, pH sensitivity and the like

Active Publication Date: 2017-04-19
南京泛成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, cyanobacteria protein has the problem of poor stability, especially it is sensitive to pH, and it is relatively stable only in a specific range of 5.5 to 7.0, because its chemical structure contains unsaturated double bonds and other oxidizable groups. Under the action of oxygen, it will oxidize and fade; and natural pigments are also very sensitive to factors such as light, temperature, and metal ions

Method used

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  • Phycocyanin with high stability as well as preparation method, composition and application thereof
  • Phycocyanin with high stability as well as preparation method, composition and application thereof
  • Phycocyanin with high stability as well as preparation method, composition and application thereof

Examples

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Effect test

Embodiment 1

[0060] Step 1: Soak spirulina powder in water for 10 hours (mass ratio of spirulina powder to water is 1:120), freeze at -30°C for 3 hours, then thaw at 15°C, and repeat freezing and thawing 3 times After centrifuging the obtained solution, collecting the supernatant is the crude extract containing phycocyanin;

[0061] The 2nd step, add sodium hydroxymethyl cellulose and ammonium sulfate to the crude extract obtained in step 1 (the addition weight of ammonium sulfate is 10% of the crude extract, and the addition weight of sodium hydroxymethyl cellulose is 10% of the crude extract. 22%), after shaking and mixing, perform ultrasonic treatment, the ultrasonic action time is 20min, the ultrasonic frequency range is 20kHz, and the ultrasonic power range is 15W / cm 2 , and collect the upper phase after standing still;

[0062] In the 3rd step, the upper phase obtained in the second step is dialyzed in a dialysis membrane with a relative molecular weight cut-off of 5000 to obtain a ...

Embodiment 2

[0067] Step 1: Soak spirulina powder in water for 12 hours (mass ratio of spirulina powder to water is 1: 180), freeze at -30°C for 4 hours, then thaw at 20°C, repeat freezing and thawing 4 times After centrifuging the obtained solution, collecting the supernatant is the crude extract containing phycocyanin;

[0068] The 2nd step, add sodium hydroxymethyl cellulose and ammonium sulfate to the crude extract obtained in step 1 (the addition weight of ammonium sulfate is 20% of the crude extract, and the addition weight of sodium hydroxymethyl cellulose is 20% of the crude extract. 26% of 26%), after oscillating and mixing, perform ultrasonic treatment, the ultrasonic action time is 30min, the ultrasonic frequency range is 40kHz, and the ultrasonic power range is 40W / cm 2 , and collect the upper phase after standing still;

[0069] In the 3rd step, the upper phase obtained in the 2nd step is dialyzed for 20 hours in a dialysis membrane with a relative molecular weight cut-off of...

Embodiment 3

[0074] Step 1: Soak spirulina powder in water for 11 hours (mass ratio of spirulina powder to water is 1:150), freeze at -30°C for 3 hours, then thaw at 16°C, repeat freezing and thawing 4 times After centrifuging the obtained solution, collecting the supernatant is the crude extract containing phycocyanin;

[0075] The 2nd step, add sodium hydroxymethyl cellulose and ammonium sulfate in the crude extract obtained in step 1 (the addition weight of ammonium sulfate is 15% of the crude extract, and the addition weight of sodium hydroxymethyl cellulose is the crude extract 24%), after shaking and mixing, perform ultrasonic treatment, the ultrasonic action time is 25min, the ultrasonic frequency range is 30kHz, and the ultrasonic power range is 30W / cm 2 , and collect the upper phase after standing still;

[0076] In the 3rd step, the upper phase obtained in the 2nd step is dialyzed for 15 hours in a dialysis membrane with a relative molecular weight cut-off of 5000 to obtain a ph...

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Abstract

The invention relates to phycocyanin with high stability as well as a preparation method, a composition and an application thereof, and belongs to the technical field of natural pigment preparation. The invention provides stable phycocyanin with low sensitivity to pH value. Crude proteins are extracted by virtue of an aqueous two-phase extraction technology, and then a part of proteins in the crude proteins is modified through a protein separation technology to improve the pH stability thereof, and the phycocyanin with high stability can be obtained after mixing the modified proteins with the other part of proteins. In addition, the invention further provides a composition containing the abovementioned phycocyanin as well as an application of the abovementioned phycocyanin in foods, feeds, cosmetics or pharmaceutical preparations.

Description

technical field [0001] The invention relates to a phycocyanin with good stability, its preparation method, composition and application, and belongs to the technical field of natural pigment preparation. Background technique [0002] Spirulina (Spirulina) contains extremely rich nutrients and is recommended by the Food and Agriculture Organization of the United Nations as "the most ideal food for human beings tomorrow". Its protein content is as high as 50-70%, and it is an excellent protein source. The content of Phycocyanin (PC) is the highest, which can reach 25-28% of the subcellular weight, which is higher than that of ordinary cyanobacteria. Phycocyanin, also known as phycocyanin, is a porphyrin-like pigment protein in blue-green algae. It is a kind of auxiliary photosynthetic pigment in cyanobacteria, red algae and cryptophyta. It is a bile protein extracted from algae such as spirulina and anabaena. It participates in photosynthesis and is also the main source of its...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/36C07K1/34C07K1/30
CPCC07K14/795
Inventor 刘微波华寿虎樊媛媛
Owner 南京泛成生物科技有限公司
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