Reagent and detection method for detecting porcine circovirus type 2 and application

A porcine circovirus and detection method technology, which is applied in the field of porcine circovirus type 2 detection, can solve the problems of lack of porcine circovirus RPA technology research and reporting, etc., achieve the effect of low hardware equipment requirements and ensure production safety
CN106566896AInactive Publication Date: 2017-04-19SHENZHEN AUDAQUE DATA TECH
3 Cites 11 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENZHEN AUDAQUE DATA TECH
Publication Date
2017-04-19
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a reagent and a detection method for detecting porcine circovirus type 2 and an application. The reagent for detecting the porcine circovirus type consists of a primer pair for conducting amplification detection on recombinant polymerase of the porcine circovirus type 2 and a probe, wherein the sequence of a forward primer of the primer pair is shown as Seq ID No.1, the sequence of a reverse primer of the primer pair is shown as Seq ID No.2 and the sequence of the probe is shown as Seq ID No.3; the 31st base of the probe is marked by BHQ1, the 33rd base is substituted by tetrahydrofuran, the 35th base is marked by FAM and the 3' terminal of the probe is modified by a phosphate group. According to the reagent for detecting the porcine circovirus type 2, a novel detecting scheme and a novel detecting approach are provided for the detection of the porcine circovirus type 2. The porcine circovirus type 2 RPA detection method based on the reagent provided by the invention is rapid and accurate, and low in requirement on hardware equipment; and the method is applicable to on-site rapid detection and is especially suitable for such departments as inspection and quarantine and the like.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The application relates to the field of porcine circovirus type 2 detection, in particular to a reagent for detection of porcine circovirus type 2, a detection method based on the reagent, and an application thereof. Background technique

[0002] According to pathogenicity and genomic differences, porcine circovirus (PCV) can be divided into non-pathogenic porcine circovirus type 1 (abbreviated PCV1) and pathogenic porcine circovirus type 2 (abbreviated PCV2). PCV2 is the main pathogen of porcine circus disease, which can cause post-weaning multisystem wasting syndrome (PMWS) in piglets. . In addition, PCV2 is also associated with porcine dermatitis and nephritis syndrome (PDNS), porcine respiratory disease complex (PRDC), reproductive disorders, and enteritis. Once the disease breaks out, it spreads very fast, and it is an important pig disease that causes serious economic losses to the world's pig industry.

[0003] In 2000, my country reported P...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More