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Reagent and detection method for detecting porcine circovirus type 2 and application

A porcine circovirus and detection method technology, which is applied in the field of porcine circovirus type 2 detection, can solve the problems of lack of porcine circovirus RPA technology research and reporting, etc., achieve the effect of low hardware equipment requirements and ensure production safety

Inactive Publication Date: 2017-04-19
SHENZHEN AUDAQUE DATA TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant RPA technology research and report on the detection of porcine circovirus type 2

Method used

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  • Reagent and detection method for detecting porcine circovirus type 2 and application
  • Reagent and detection method for detecting porcine circovirus type 2 and application
  • Reagent and detection method for detecting porcine circovirus type 2 and application

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Embodiment

[0024] 1. Materials and methods

[0025] 1. Tested strains and reagents

[0026] Porcine circovirus type 2 (PCV2), pseudorabies virus (PRV), swine fever virus (CSFV), swine vesicular disease virus (SVDV), vesicular stomatitis virus (VSV) and porcine circovirus 1 used in this example Type (PCV1), foot-and-mouth disease virus type O, foot-and-mouth disease virus Asia type I and African swine fever virus (ASFV) and other inactivated strains were all obtained by the Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry-Exit Inspection and Quarantine Bureau (hereinafter referred to as "Shenzhen Inspection and Quarantine Bureau"). Center for Animals and Plants") provided and deposited.

[0027] The recombinant plasmid pMD18T-PCV2 containing the RPA detection target gene of PCV2 was constructed and preserved by the Animal and Plant Center of Shenzhen Inspection and Quarantine Bureau. Escherichia coli TOP10 competent cells were prepared and preserved by the ...

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Abstract

The invention discloses a reagent and a detection method for detecting porcine circovirus type 2 and an application. The reagent for detecting the porcine circovirus type consists of a primer pair for conducting amplification detection on recombinant polymerase of the porcine circovirus type 2 and a probe, wherein the sequence of a forward primer of the primer pair is shown as Seq ID No.1, the sequence of a reverse primer of the primer pair is shown as Seq ID No.2 and the sequence of the probe is shown as Seq ID No.3; the 31st base of the probe is marked by BHQ1, the 33rd base is substituted by tetrahydrofuran, the 35th base is marked by FAM and the 3' terminal of the probe is modified by a phosphate group. According to the reagent for detecting the porcine circovirus type 2, a novel detecting scheme and a novel detecting approach are provided for the detection of the porcine circovirus type 2. The porcine circovirus type 2 RPA detection method based on the reagent provided by the invention is rapid and accurate, and low in requirement on hardware equipment; and the method is applicable to on-site rapid detection and is especially suitable for such departments as inspection and quarantine and the like.

Description

technical field [0001] The application relates to the field of porcine circovirus type 2 detection, in particular to a reagent for detection of porcine circovirus type 2, a detection method based on the reagent, and an application thereof. Background technique [0002] According to pathogenicity and genomic differences, porcine circovirus (PCV) can be divided into non-pathogenic porcine circovirus type 1 (abbreviated PCV1) and pathogenic porcine circovirus type 2 (abbreviated PCV2). PCV2 is the main pathogen of porcine circus disease, which can cause post-weaning multisystem wasting syndrome (PMWS) in piglets. . In addition, PCV2 is also associated with porcine dermatitis and nephritis syndrome (PDNS), porcine respiratory disease complex (PRDC), reproductive disorders, and enteritis. Once the disease breaks out, it spreads very fast, and it is an important pig disease that causes serious economic losses to the world's pig industry. [0003] In 2000, my country reported P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 曾少灵曹琛福叶奕优刘建利王潇林彦星张彩虹吕建强杨俊兴花群义
Owner SHENZHEN AUDAQUE DATA TECH
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