Specific tumor probe region design method and device and probe for target region capture high-throughput sequencing

Abstract

The invention discloses a specificity tumor probe area designing method for acquiring high-throughput sequencing in a target area, a device and a probe. The method comprises steps of calculating a gene and mutation related to a to-be-designed tumor in a first tumor mutation database, looking for a CDS area having the highest mutation frequency and designating the area as a first area, verifying the first area via tumor patient data stored in a second tumor mutation database, maximizing a sample frequency to achieve an area containing the most patients and designing the area as a second area, and calculating sample frequency and a RI value, further optimizing the sample area to achieve a third area. The RI value represents an average mutation number contained in each 1kb. By the use of the method, research is only required for the target area, so sequencing range can be greatly reduced and sequencing cost and time can be reduced; detection period can be obviously reduced; and cost can be lowered, so the specificity tumor probe area designing method for acquiring high-throughput sequencing in the target area is more suitable for clinical application.

Description

technical field [0001] The present invention relates to the technical field of tumor probe design, in particular to a specific tumor probe region design method and device for target region capture high-throughput sequencing and a probe. Background technique [0002] According to the new edition of the "World Cancer Report" released by the World Health Organization (WHO) on February 4, 2014, the number of new cancer patients is about 14 million each year, and it is expected to increase to 19 million in 2025 and 22 million in 2030. Grow to 24 million in 2035. Among them, the number of cancer cases in China in 2012 was 3.065 million, accounting for about one-fifth of the global cases. The number of cancer deaths was 2.205 million, accounting for about a quarter of the global cancer deaths. In the next 20 years, the incidence and death of cancer in my country will continue to rise. According to the forecast of the International Agency for Research on Cancer, if no effective m...

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Problems solved by technology

There are certain defects in this detection. The design of the gene chip is not targeted, and the cost is relatively high. It is impossible to conduct large-scale research on liver cancer patients.

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