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High purity and high survival rate live ant acquisition method

A collection method and technology of purity, applied in the field of biology, can solve the problems of time-consuming and laborious collection methods and low purity, and achieve the effects of ensuring food or medicinal safety, improving survival rate, and avoiding microbial contamination.

Inactive Publication Date: 2017-04-26
HUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The collection methods of the above two kinds of medicinal ants are time-consuming and labor-intensive, and the surface of the insect body will adhere to soil or microorganisms, and the purity is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] For an RIFA drug efficacy test, individual RIFAs need to be placed in jars and administered with drugs. The test preparations are as follows:

[0028] (1) Apply a layer of talcum powder on the edge of the plastic bucket, dig out the whole ant nest with a shovel and put it in the plastic bucket;

[0029] (2) Put multiple small wooden branches on the top of the ant nest;

[0030] (3) Slowly add water to the plastic bucket so that the water is 1 / 10 from the top of the ant nest, and the speed of adding water should be slow so that the live ants can evacuate upwards in time, and wait for about 1 hour;

[0031] (4) After the live ants gather on the wooden branches at the top of the ant nest, gently put the wooden branches full of live ants into the clean 2L milk powder tank prepared in advance with a long branch, cover it well, and put it in a upper protective bag;

[0032] (5) Take the milk powder can to the laboratory, quickly open the lid, and drop 2 drops of ether into...

Embodiment 2

[0036] To collect wild Pseudomonas spiny ants, the method is as follows:

[0037] (1) Apply a layer of talcum powder on the edge of the plastic bucket, dig out the whole ant nest with a shovel and put it in the container;

[0038] (2) Put multiple small wooden branches on the top of the ant nest;

[0039] (3) Slowly add water to the plastic bucket, so that the water is added to 1 / 10~2 / 10 from the top of the ant nest, and the speed of adding water should be slow so that the live ants can evacuate upward in time;

[0040] (4) After the live ants gather on the wooden branches at the top of the ant nest, use a long branch to gently put the wooden branches full of live ants into the clean 2L milk powder tank prepared in advance, cover it well, and put it on protective bag;

[0041] (5) Take the milk powder tank to the operating room, quickly open the lid, and drop 3 drops of ether into the container;

[0042] (6) When the black spiny ants are stunned, immediately shake it off th...

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PUM

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Abstract

The invention belongs to the field of biology, and especially relates to a biological acquisition method. The method comprises: firstly, using the habit that live ants fear water, slowing filling water into a container which holds an ant nest, to separate the live ants from the ant nest, to obtain high purity live ants, and then using the characteristic that diethyl ether and other volatile general anesthesia liquid drugs can dizzy creatures by low dosage, and putting the live ant individuals into the clean container under the condition of swoon. The method can obtain high purity and high survival rate live ants, and is suitable for acquisition of red imported fire ants, white ants, and polyrhachis dives, and other medical ants.

Description

technical field [0001] The invention belongs to the field of biology, in particular to a biological collection method. Background technique [0002] Red fire ants and termites are two kinds of organisms that are seriously harmful to humans. Both of these creatures live in underground ant nests, each containing hundreds of thousands to millions of individuals, and are social insects. [0003] When conducting an ant-killing efficacy test on red imported fire ants or termites in the laboratory, experimenters usually use a brush to gently pick live red imported fire ants or termites from the ant nest one by one, and put them into wide-mouthed glass bottles. Drugs were administered for observation. However, because live red fire ants or termites can crawl freely, it takes time and effort to pick them into the jar, and the insect body is soft, so it is easy to crush the insect body to death if you are not careful, or when you pick it into the jar, the insect body will be damaged...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01M1/20A01M1/10
CPCA01M1/103A01M1/2027
Inventor 林芳花陈志光陈洁萍陈鹤廷陈捷阳
Owner HUIZHOU UNIV
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