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SiRap2b-GNs coupling compound for targeted therapy of malignant tumor and preparation method of siRap2b-GNs coupling compound

A technology for targeted therapy and malignant tumor, applied in the field of biomedicine, can solve the problems of p53 inactivation and p53 activation related pathway destruction, and achieve the effect of fewer operation steps, improved tumor treatment effect, and short production cycle.

Inactive Publication Date: 2017-04-26
YANGZHOU UNIV
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Problems solved by technology

It has been reported that about 50% of human tumors have mutations in the p53 gene, and in tumors without p53 mutations, most of the pathways related to p53 activation are disrupted, resulting in inactivation of p53

Method used

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  • SiRap2b-GNs coupling compound for targeted therapy of malignant tumor and preparation method of siRap2b-GNs coupling compound
  • SiRap2b-GNs coupling compound for targeted therapy of malignant tumor and preparation method of siRap2b-GNs coupling compound
  • SiRap2b-GNs coupling compound for targeted therapy of malignant tumor and preparation method of siRap2b-GNs coupling compound

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Embodiment Construction

[0028] 1. Preparation of gold nanoshells (GNs):

[0029] Refer to the method reported in the literature (Jian You, Guodong Zhang, and Chun Li. Exceptionally high payload of doxorubicin in hollow gold nanospheres for near-infraredlight-triggered drug release. ACS Nano, 2010. 4(2): 1033-1041.), 3.4 mgAgNO 3 And 15 mg trisodium citrate dihydrate dissolved in 100 mL deionized water, add 7.6 mg NaBH while stirring 4 , Heated to 60 ℃ and stirred for 2 h. Cool to room temperature, add 83.4 mg hydroxylamine hydrochloride, stir for 5 min, then add 29.75 mg AgNO 3 , Stir for more than 2 h (preferably overnight). Then the solution was heated to 60°C, and 1 mL of 20 mg / mL chloroauric acid aqueous solution was quickly added, and the reaction was stopped after stirring at 60°C for 1 hour. Let stand at 4°C for 48 h, and discard the bottom sediment. The upper solution is the GNs, which is stored at 4°C for later use. According to the method reported by Prevo et al. (Brian G. Prevo, Shelley A....

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Abstract

The invention discloses a SiRap2b-GNs coupling compound for targeted therapy of a malignant tumor and a preparation method of the siRap2b-GNs coupling compound, and belongs to the technical field of biomedicine. The preparation method comprises the following steps: mixing a gold nano-shell solution with mPEG-SH-2000 for reaction to prepare a PEG-modified gold nano-shell; then, using DEPC water as a solvent; mixing the PEG-modified gold nano-shell with siRap2b for reaction to prepare the siRap2b-GNs coupling compound. According to the invention, the hollow nano-shell is used as a medicine carrier and can be used for the targeted therapy of malignant tumor after siRap2b is coupled; Rap2b is used as the target; the medicine can be stably and efficiently transported to the tumor tissues in a fixed direction by utilizing that the GNs has all advantages of gold nanoparticles, to achieve effective killing of tumor cells, thereby greatly improving the tumor therapy effect.

Description

Technical field [0001] The invention belongs to the technical field of biomedicine, and aims to prepare a new type of preparation for tumor treatment, and the classification number is A61P. Background technique [0002] Cancer, or malignant tumor, is a type of disease that seriously threatens human health. According to the latest data released by the International Agency for Research on Cancer (IARC), more than 8 million people die from cancer each year. By 2020, there will be more than 17 million new cancer patients worldwide each year. The number of new cancer cases in my country will increase from 300 in 2012. Million has grown to nearly 4 million, so the development of highly effective anti-tumor agents and the formulation of more effective tumor treatment programs are currently one of the urgent problems to be solved. [0003] The formation of malignant tumors is related to many factors. Among them, the abnormality of the tumor suppressor gene p53 and its signaling pathway pla...

Claims

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Application Information

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IPC IPC(8): A61K47/52A61K48/00A61K31/7088A61K47/10A61K41/00A61P35/00
CPCA61K31/7088A61K41/0052A61K47/10
Inventor 张新跃丁笠孙若男
Owner YANGZHOU UNIV
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