Method for separation and determination of metaraminol bitartrate and its isomers by high performance liquid chromatography

Abstract

The invention discloses a method for separating and detecting metaraminol bitartrate and isomers thereof through high performance liquid chromatography. The method adopts a chiral chromatographic column, and adopts an n-hexane, anhydrous ethanol and isopropylamine mixed solution as a mobile phase; a volume ratio of n-hexane to anhydrous ethanol to isopropylamine in the mobile phase is (60-95):(5-30):(0.05-0.5); and the chiral chromatographic column is a bonded chiral chromatographic column adopting silica gel with the surface covalently bonded with amylose-tris(3,5-dimethylphenylcarbamate) as a filler. Separation and detection of the metaraminol bitartrate and the isomers thereof are realized by adopting the high performance liquid chromatography and the chiral chromatographic column, especially creatively making the special mobile phase, so the effectiveness and the safety of the metaraminol bitartrate is finally guaranteed.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a method for separating and measuring metaraminol bitartrate and its isomers by high performance liquid chromatography. Background technique [0002] Metaraminol Bitartrate is the bitartrate of Metaraminol Bitartrate. It is an α-adrenoceptor agonist developed by Fresenius Kabi Company in the United States. It mainly acts on α-receptors. It is suitable for early treatment of shock and prevention of spinal Acute hypotension during internal block anesthesia. [0003] Due to the existence of two chiral carbon atoms in metaraminol tartrate, there are four configurations (1R, 2S), (1S, 2R), (1R, 2R), (1S, 2S), among which (1R, 2S) is the effective configuration, (1S, 2R) is its enantiomer, and (1R, 2R) and (1S, 2S) are its diastereomers. [0004] The four configuration structures of metaraminol are as follows: [0005] (1R, 2S) configuration: . [0006] (...

AI Technical Summary

Problems solved by technology

[0009] Although various methods are used to control the other three isomers in the preparation process of metaraminol bitartrate, there will still be a certain amount of isomers in the final metaraminol bitartrate bulk drug

[0010] The separation of optical isomers of compounds containing multiple chiral carbon atoms has always been a difficult point in the quality control of chiral drugs. However, the separation and determination of metaraminol tartrate and its isomers has not been reported in the literature so far

Images