Method for quantitative determination of PSMA key intermediate chiral isomer

A technology for quantitative determination of chiral isomers, which is applied in the field of chemical medicine and can solve the problems of low column efficiency, difficult to effectively separate chiral isomers, and effective separation of chiral isomers of difficult compounds.
CN113391017APending Publication Date: 2021-09-14SUZHOU IBIO TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUZHOU IBIO TECH CO LTD
Publication Date
2021-09-14

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Abstract

The invention provides a method for quantitative determination of a PSMA key intermediate chiral isomer. The method is a reversed-phase high performance liquid chromatography, and the chromatographic conditions are that a polysaccharide coated reversed-phase chiral column is adopted, the chiral column is CHIRALCEL OD-R, water, acetonitrile and trifluoroacetic acid in a ratio of 50: 50: 0.1 are used as a diluent, the column temperature of the chiral column is 30 DEG C, a phase A, namely a 0.1% trifluoroacetic acid aqueous solution, and a phase B, namely a 0.1% trifluoroacetic acid acetonitrile solution are used as mobile phases, the flow velocity of the mobile phases is 1.0 mL / min, and the detection wavelength of liquid chromatography is 220nm. The method has the beneficial effects that the method can efficiently and accurately realize separation and determination of the chiral isomer of the PSMA key intermediate, the chiral isomer of the PSMA key intermediate can be rapidly and accurately analyzed, the peak shape is relatively good, and the separation degree conforms to the specification so that the quality of the PSMA key intermediate is effectively controlled. The method is high in separation degree, good in specificity, high in sensitivity and easy and convenient to operate.
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Description

technical field

[0001] The invention belongs to the technical field of chemistry and medicine, and in particular relates to a method for quantitatively determining the chiral isomer of a key intermediate of PSMA. Background technique

[0002] Prostate-specific membrane antigen (PSMA) is a type II cell surface membrane-bound glycoprotein with a molecular weight of about 110kD, including an intracellular fragment (amino acid sequence 1-18), a transmembrane domain (amino acid sequence 19-43), and an extracellular domain (amino acid sequence 44-750). The intracellular fragment and the transmembrane domain are now considered to have no significant role, while the extracellular domain is involved in a variety of specific activities. PSMA plays a very important role in the nervous system, it promotes the metabolism of N-acetylaspartamine (NAAG) and generates glutamic acid and N-acetylaspartic acid. Correspondingly, it is also sometimes related to N-acetyl α-linked acid dipeptidas...

Claims

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