Method for measuring ancient cowhair micro-trace based on proteomics

Abstract

The invention discloses a method for measuring ancient cowhair micro-trace based on proteomics. A soil sample of the ancient cowhair micro-trace, a soil sample of a surface soil layer and a soil sample of a raw soil layer are extracted and separated, protein is effectively separated by a two-dimensional electrophoretic technique, the electrophoretic results of the three samples are compared, the influence that the soil contains protein and the interference of external factors are excluded, stripes in the electrophoretic results are cut off and subjected to in-gel digestion, mass spectrometric analysis is conducted, and protein identification can be realized by comparing the mass spectrometric analysis result with the amino acid sequence in a protein database. The ancient cowhair micro-trace is measured by a proteomic method, the problem that the common analysis test is helpless during analysis of micro-trace residues is solved, the protein varieties of the samples are obtained accurately through proteomic measurement, and whether the micro-trace sample is the cowhair micro-trace is judged, so that on one hand, the method is high in sensitivity and is simple and convenient to operate, and on the other hand, the use amount of the samples is small.

Description

technical field [0001] The invention relates to a method for measuring microtraces of ancient cow hair, more specifically relates to a method for measuring microtraces of ancient cow hair based on proteomics. Background technique [0002] Cow hair is a natural animal hair fiber with keratinous tissue, which is tough and elastic. It can be used to produce various wool fabrics and is the main raw material for wool spinning industry. [0003] China has a long history. A large number of cow hair products have been unearthed from early Chinese tombs. The main component of these cow hair and cow hair products is protein, which has been buried in the underground environment for a long time. Under the influence of factors such as humidity, heat and microorganisms, the fibers will change. degree of degradation; after unearthed, due to drastic changes in environmental conditions, the aging of fibers was further accelerated. Therefore, in the long course of history, some cow hair and i...

AI Technical Summary

Problems solved by technology

[0003] China has a long history. A large number of cow hair products have been unearthed from early Chinese tombs. The main component of these cow hair and cow hair products is protein, which has been buried in the underground environment for a long time. Under the influence of factors such as humidity, heat and microorganisms, the fibers will change. degree of degradation; after unearthed, due to drastic changes in environmental conditions, the aging of fibers was further accelerated. Therefore, in the long course of history, some cow hair and its products buried in tombs or ruins had already lost their physical appearance and degraded into Peptides and amino acids, either become traces or melt into the soil, are no longer identifiable to the naked eye, and the older the evidence is, the harder it is to find, which brings great difficulties to the identification and protection of cattle hair cultural relics

[0004] At present, ordinary analysis and detection techniques, such as Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction analysis, etc., are often at a loss when encountering such micro-trace residue analysis. For the identification of cow hair micro-traces at home and abroad, Most of them still stay in the analysis of fiber morphology, and the identification results obtained are not completely accurate; therefore, it is very necessary to find a more scientific method to identify the micro-traces of cow hair