Method for measuring ancient cowhair micro-trace based on proteomics

A proteomics and protein technology, applied in the preparation of test samples, biological testing, measurement devices, etc., can solve problems such as traces, fiber aging, and melting into soil, etc., to achieve less sample usage, easy operation, and accuracy. high degree of effect

Active Publication Date: 2017-04-26
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] China has a long history. A large number of cow hair products have been unearthed from early Chinese tombs. The main component of these cow hair and cow hair products is protein, which has been buried in the underground environment for a long time. Under the influence of factors such as humidity, heat and microorganisms, the fibers will change. degree of degradation; after unearthed, due to drastic changes in environmental conditions, the aging of fibers was further accelerated. Therefore, in the long course of history, some cow hair and its products buried in tombs or ruins had already lost their physical appearance and degraded into Peptides and amino acids, either become traces or melt into the soil, are no long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: a kind of method based on proteomics determination ancient cattle hair microtrace, this method adopts the following steps:

[0063] (A) Weigh the ancient cow hair micro-trace soil sample, 25g each of the surface soil sample and the soil layer soil sample, grind three groups of samples with a mortar, and mark them as three groups of A, B and C;

[0064] (B) preparation mass fraction is 3000ml of potassium hydrogen peroxomonosulfate compound saline solution 3000ml, adjusts this solution with saturated sodium hydroxide, so that the pH of solution is 4, is divided into three groups of equal amounts for subsequent use;

[0065] (C) Put the three groups of samples in step (A) into the solution prepared in step (B) respectively according to the bath ratio of 1:40, and then stir at 30°C for 40min, and the three groups of experiments were performed independently under the same conditions operate;

[0066] (D) Use a high-speed refrigerated centrifuge to centrifuge ...

Embodiment 2

[0080] Embodiment 2: a kind of method based on proteomics measures ancient cow hair microtrace, the method adopts following steps:

[0081] (A) Weigh the ancient ox hair micro-trace soil sample, 27g each of the surface soil sample and the soil layer soil sample, grind three groups of samples respectively with a mortar, and mark them as three groups of A, B and C;

[0082] (B) preparation mass fraction is 4200ml of potassium hydrogen persulfate complex salt solution 4200ml, with saturated sodium hydroxide, this solution is adjusted, so that the pH of solution is 5, is divided into three groups of equal amounts for subsequent use;

[0083] (C) Put the three groups of samples in step (A) into the solution prepared in step (B) respectively according to the bath ratio of 1:50, and then stir at 40°C for 45min, and the three groups of experiments were performed independently under the same conditions operate;

[0084] (D) Use a high-speed refrigerated centrifuge to centrifuge the th...

Embodiment 3

[0098] Embodiment 3: a kind of method based on proteomics measures ancient cow hair microtrace, the method adopts the following steps:

[0099] (A) Weigh the ancient ox hair micro-trace soil sample, 30g each of the surface soil sample and the soil layer soil sample, grind three groups of samples respectively with a mortar, and mark them as three groups of A, B and C;

[0100] (B) preparation mass fraction is 5400ml of potassium hydrogen persulfate complex salt solution 5400ml, with saturated sodium hydroxide, this solution is adjusted, so that the pH of solution is 6, is divided into three groups for subsequent use in equal amounts;

[0101] (C) Put the three groups of samples in step (A) into the solution prepared in step (B) respectively according to the bath ratio of 1:60, and then stir at 50°C for 50min, and the three groups of experiments were performed independently under the same conditions operate;

[0102] (D) Centrifuge the three groups of samples finally obtained t...

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Abstract

The invention discloses a method for measuring ancient cowhair micro-trace based on proteomics. A soil sample of the ancient cowhair micro-trace, a soil sample of a surface soil layer and a soil sample of a raw soil layer are extracted and separated, protein is effectively separated by a two-dimensional electrophoretic technique, the electrophoretic results of the three samples are compared, the influence that the soil contains protein and the interference of external factors are excluded, stripes in the electrophoretic results are cut off and subjected to in-gel digestion, mass spectrometric analysis is conducted, and protein identification can be realized by comparing the mass spectrometric analysis result with the amino acid sequence in a protein database. The ancient cowhair micro-trace is measured by a proteomic method, the problem that the common analysis test is helpless during analysis of micro-trace residues is solved, the protein varieties of the samples are obtained accurately through proteomic measurement, and whether the micro-trace sample is the cowhair micro-trace is judged, so that on one hand, the method is high in sensitivity and is simple and convenient to operate, and on the other hand, the use amount of the samples is small.

Description

technical field [0001] The invention relates to a method for measuring microtraces of ancient cow hair, more specifically relates to a method for measuring microtraces of ancient cow hair based on proteomics. Background technique [0002] Cow hair is a natural animal hair fiber with keratinous tissue, which is tough and elastic. It can be used to produce various wool fabrics and is the main raw material for wool spinning industry. [0003] China has a long history. A large number of cow hair products have been unearthed from early Chinese tombs. The main component of these cow hair and cow hair products is protein, which has been buried in the underground environment for a long time. Under the influence of factors such as humidity, heat and microorganisms, the fibers will change. degree of degradation; after unearthed, due to drastic changes in environmental conditions, the aging of fibers was further accelerated. Therefore, in the long course of history, some cow hair and i...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N27/447G01N1/28
CPCG01N1/286G01N27/447G01N33/6848G01N2001/2866
Inventor 彭志勤古锦翠王秉万军民胡智文
Owner ZHEJIANG SCI-TECH UNIV
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