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Deglycosylated holothurian secondary saponin and preparation method thereof

A technology for secondary saponins and sea cucumber saponins is applied in the field of enzymatic hydrolysis and preparation of sea cucumber saponins, which can solve the problems of less enzymatic hydrolysis of sea cucumber saponins, many side reactions, poor specificity and the like, and achieves improved biological activity, single product, and reduced toxicity. Effect

Active Publication Date: 2017-05-10
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are very few related studies on the enzymatic hydrolysis of sea cucumber saponins, so the enzymatic hydrolysis method in Gao Juan's paper has very good reference and guiding significance for the study of the enzymatic hydrolysis of sea cucumber saponins by glycosidases
Common chemical methods are used to hydrolyze saponins, but they have the disadvantages of many side reactions, miscellaneous products, and poor specificity.

Method used

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  • Deglycosylated holothurian secondary saponin and preparation method thereof
  • Deglycosylated holothurian secondary saponin and preparation method thereof
  • Deglycosylated holothurian secondary saponin and preparation method thereof

Examples

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Effect test

Embodiment 1

[0041] (1) Put the purchased dried sea cucumber into an oven at 50°C, take it out after 2 days, and crush it into powder;

[0042] (2) Take dried sea cucumber powder (Philippine sea cucumber about 1 kg), extract it with 60% ethanol for 7 days, stir continuously during this period, extract 3 times, evaporate under reduced pressure to obtain extract paste, and freeze-dry to obtain crude extract;

[0043] (3) Dissolve the above crude extract fully with a small amount of water first, and then extract five times with water-saturated n-butanol. The first time extracts the sample and water-saturated n-butanol 1:3 (v / v), and then extracts it twice with water-saturated n-butanol. 1:2 (v / v) extraction, the last two extractions were 1:1 (v / v). Concentrate the supernatant under reduced pressure to obtain extract extract, and evaporate to dryness in a 60°C water bath to obtain crude saponin powder;

[0044] ⑷, enrich the thick saponin in step ⑶ with macroporous resin column chromatography...

Embodiment 2

[0053] (1) Put the purchased dried sea cucumber into an oven at 50°C, take it out after 2 days, and crush it into powder;

[0054] (2) Take dried sea cucumber powder (Philippine sea cucumber about 1kg), extract it with 60% ethanol for 7 days, keep stirring during the period, extract 3 times, evaporate under reduced pressure to obtain extract paste, freeze dry in a freeze dryer to obtain crude extract ;

[0055] (3) Dissolve the above crude extract fully with a small amount of water first, and then extract five times with water-saturated n-butanol. The first time extracts the sample and water-saturated n-butanol 1:3 (v / v), and then extracts it twice with water-saturated n-butanol. 1:2 (v / v) extraction, the last two extractions were 1:1 (v / v). Concentrate the supernatant under reduced pressure to obtain extract extract, and evaporate to dryness in a 60°C water bath to obtain crude saponin powder;

[0056] ⑷, enrich the thick saponin in the step ⑶ with macroporous resin column ...

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Abstract

The invention provides deglycosylated holothurian secondary saponin and a preparation method of the deglycosylated holothurian secondary saponin. For the deglycosylated holothurian secondary saponin, two rear glycosyl radicals on carbohydrate chains of holothurian A (HA) and Echinoside A (EA) are removed through the enzymolysis of glycosidase, and thus the secondary saponin is obtained. The preparation method of the secondary saponin comprises the following steps: alcohol digestion, crude extraction adopting macroporous resin, and purification adopting a silicagel column, thus HA and EA are obtained, finally, enzymolysis is carried out by adopting glycosidase, and thus deglycosylated HA secondary saponin and EA secondary saponin are obtained respectively. According to the deglycosylated holothurian secondary saponin and the preparation method of the deglycosylated holothurian secondary saponin, through the used glycosidase, holothurian saponin forms secondary saponin through enzymolysis under the mild condition, so that the toxicity of holothurian saponin is reduced, and the biological activity of holothurian saponin is improved; meanwhile, glycosidase is adopted for enzymolysis of holothurian saponin, so that the side reaction and by-products brought by micro-biological degradation or degradation adopting crude enzyme extracted from microbes are avoided, the purity and yield of the enzymolysis products are improved, the single product can be obtained, and the purity can achieve 100%.

Description

technical field [0001] The invention relates to an enzymatic hydrolysis preparation method of sea cucumber saponins, in particular to a method for enzymatic hydrolysis of sea cucumber saponins with glycosidase, and finally obtains sea cucumber secondary saponins from which two sugar groups have been removed. Background technique [0002] Sea cucumbers belong to the phylum Echinodermata, the class Holothuria, and the order Chiroptera. Sea cucumber saponins are important secondary metabolites and active substances in sea cucumbers. The main active functions of sea cucumber saponins are anti-tumor, anti-fungal, anti-virus, anti-aging and hemolysis, as well as regulating the growth and development of specific cells and immunity. adjustment etc. Therefore, sea cucumber saponins are an important indicator of the medicinal value of sea cucumbers. [0003] Saponins have a hemolytic effect, and saponins can combine with red blood cell membranes to form sterol complexes, leading to ...

Claims

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Application Information

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IPC IPC(8): C12P33/20
CPCC12P33/20
Inventor 陈静兰李兆杰王静凤王玉明薛勇薛长湖
Owner OCEAN UNIV OF CHINA
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