Gene detection kit for prognosing gastric cancer metastasis and use method of gene detection kit

Abstract

The invention relates to a gene detection kit for prognosing gastric cancer metastasis. The kit comprises a DNA library building kit, wherein the DNA library building kit comprises a high-risk gene probe and a low-risk gene probe; the high-risk gene probe comprises CDH1, CDH2, SNAIL, SLUG, MUC4, MUC6, PRSS3, USP6, MLH1, MSH2, MSH6, PMS2, TGFBR2, MMP2, MMP9, BRCA1, BRCA2, PALB2, ATM, ATR, MUTYH, EMSY, ERCC4, RAD51, PARP1 and XRCC1; the low-risk gene probe comprises ATRX, BRIP1, FANCA, FANCB, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL, FANCM, FANCO, FANCP, MDM2, MDM4, MLH1, NPM1, PP2R1A, PRKDC, RAD50, STAG2, XRCC5 and CRCC6. The invention further discloses a use method of the kit. The use method comprises the following steps: extracting cfDNA in a blood sample; building a library for the cfDNA through the DNA library building kit, and then sequencing the DNA to obtain a gene overall length sequence; carrying out gene mutation analysis on the gene overall length sequence.

Description

technical field [0001] The invention relates to the field of gene diagnosis kits, in particular to a gene detection kit for predicting gastric cancer metastasis and its application method. Background technique [0002] Cancer death is currently a major factor threatening human life and health. More than 90% of cancer patients die from tumor metastasis, and the survival period of cancer patients with metastasis generally does not exceed five years. In my country, the incidence of gastric cancer ranks second among malignant tumors, and the mortality rate ranks fourth. More than 90% of hospitalized cases of gastric cancer in my country are advanced gastric cancer. Even if radical treatment is performed, the five-year survival rate is still hovering around 30%. The main reason affecting the prognosis of gastric cancer is metastasis. Gastric cancer metastasis mostly occurs in the middle and late stage of patients, and only a small part occurs in the early stage of gastric cancer...

AI Technical Summary

Problems solved by technology

[0009] 1) Radionuclide bone scan (ECT): the diagnosis rate of gastric cancer bone metastases is relatively high, and metastases can be detected several months before bone metastases are found on X-ray plain film and CT, and the diagnosis of multiple gastric cancer bone metastases is false positive Rarely, but there is a certain false positive for the diagnosis of solitary gastric cancer bone metastasis;

[0010] 2) X-ray examination: it can be used as a basic examination method for the diagnosis of gastric cancer bone metastasis, it is helpful to detect the degree of destruction of bone marrow and cortical bone at the site of osteolytic bone metastasis, and it can also be used to confirm other imaging findings. Destroyed metastases have low sensitivity;

However, because of its high price, it cannot be widely used at present, and can only be used as a special inspection method.

[0019] At present, medical imaging is mainly used clinically for the clinical diagnosis of gastric cancer metastasis, but these methods also have some obvious shortcomings, mainly reflected in: the indicators are not objective enough, and cannot provide a clear judgment for micrometastases and critical conditions; The identified metastases are already in the middle and advanced stage for the patient, and the best treatment opportunity is missed, which is not conducive to the treatment of the patient

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