The invention discloses an ultrasonic RF (radio frequency) time sequence-based tissue characterization method, which comprises the following steps of 1, feature extraction: performing operations as shown as follows on each tissue sample to obtain corresponding feature vectors; 2, tissue recognition, wherein the step1 comprises the following steps: 1-1, scanning tissues to acquire multiple frames of ultrasonic echo RF signals of the tissues; 1-2, demodulating a certain ultrasonic echo RF signal and displaying a B-mode graph; 1-3, selecting an ROI (region of interest) of which the size is M*N from the B-mode graph; 1-4, taking previous L RF signals from each point in the ROI to construct M*N ultrasonic RF time sequences of which the length is L; 1-5, extracting fractal features, frequency domain features and time domain features of the ultrasonic RF time sequences to obtain the feature vectors of each tissue samples. According to the ultrasonic RF time sequence-based tissue characterization method disclosed by the invention, the features from three angles: fractal, frequency domain and time domain, are extracted based on the ultrasonic RF time sequences, and the tissues are recognized by using a classifier; the ultrasonic RF time sequence-based tissue characterization method can be widely applied to the field of ultrasonic medical detection and ultrasonic tissue characterization, and the like.