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Labeled substances and their applications in immunology, analytes and test strips

A technology for labeling substances and analytes, which is applied in the field of labeling substances and its application in immunology, analytes and test strips, and can solve problems such as difficult antigens or antibodies, damage to the surface state of latex particles, and non-specific aggregation of latex particles , to achieve the effect of excellent durability and visibility

Active Publication Date: 2021-08-03
NIPPON STEEL CHEM &MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the color tone of the colloidal particles is determined by the particle size and preparation conditions, so there is a problem that it is difficult to obtain a desired clear and deep color tone, that is, the visibility is insufficient.
[0008] In addition, the above-mentioned colored latex particles have a problem that the coloring effect by the pigment is low and the visual judgment is not sufficient.
Furthermore, if the coloring amount of the pigment is increased in order to solve the above problem, the pigment covers the surface of the latex, and the original surface state of the latex particles is damaged, so there is a problem that it is difficult to bind the antigen or the antibody.
In addition, there are also problems such as clogging in the pores of chromatography media such as membrane filters, non-specific aggregation of latex particles, or intensive coloring due to increased coloring of pigments, which does not necessarily lead to improvement in performance.
[0014] As described above, latex particles bonded or coated with gold nanoparticles are expected as reagents for immunological measurements, but in the prior art, durability and visibility are not sufficient.
In addition, even with high visibility, applicable applications and usage environments are limited

Method used

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  • Labeled substances and their applications in immunology, analytes and test strips
  • Labeled substances and their applications in immunology, analytes and test strips
  • Labeled substances and their applications in immunology, analytes and test strips

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0201]

[0202] After dissolving Aliquat 336 (manufactured by Aldrich) (1.00 g) and polyethylene glycol methyl ethyl ether methacrylate (PEGMA, 2.00 g) in 80 g of pure water , 2-vinylpyridine (2-VP, 9.90 g) and divinylbenzene (DVB, 0.100 g) were added, and stirred at 250 rpm and 60° C. for 30 minutes under a nitrogen stream. After stirring, 2,2-azobis(2-methylpropionamidine) dihydrochloride (AIBA, 0.100 g) dissolved in 9.00 g of pure water was added dropwise over 5 minutes, and stirred at 250 rpm and 60°C for 6 minutes. hour, thus obtaining resin particles with an average particle diameter of 0.45 μm. The resin particles were precipitated by centrifugation (9000 rpm, 10 minutes), and the supernatant was removed, and then dispersed in pure water again to obtain a 10 wt % resin particle dispersion.

[0203]

[0204] A 30 mM chloroauric acid aqueous solution (80 g) was added to the resin particle dispersion (3.05 g), and the mixture was left to stand at room temperature for ...

Embodiment 2

[0206] Except adding 10mM chloroauric acid aqueous solution (56g) to the resin particle dispersion (3.05g) obtained in Example 1, obtain the gold ion adsorption resin particle dispersion, average particle diameter with the same method as Example 1 0.6μm resin-gold composite and 1wt% resin-gold composite dispersion. The absorbance of the resin-gold complex in the resin-gold complex dispersion liquid was 1.04. In addition, the average particle diameter of the gold particles in the resin-gold composite was 7.61 nm, and the supported amount of gold was 36.8 wt%.

Embodiment 3

[0208]

[0209] 2-VP (9.90g) and DVB (0.100g) were added to 450g of pure water, and it stirred at 250 rpm and 60 degreeC for 30 minutes under nitrogen flow. After stirring for 30 minutes, AIBA (0.100 g) dissolved in 9.00 g of pure water was dropped over 5 minutes, and stirred at 250 rpm for 6 hours to obtain resin particles having an average particle diameter of 0.10 μm. The resin particles were precipitated by centrifugation (9000 rpm, 20 minutes), and after removing the supernatant, they were dispersed in pure water again to obtain a 10 wt % resin particle dispersion.

[0210]

[0211] A 30 mM chloroauric acid aqueous solution (198 g) was added to the resin particle dispersion (5.0 g), and it was left to stand at room temperature for 24 hours. Thereafter, utilize centrifugation (3000rpm, 10 minutes) to make the resin particles precipitate, and remove the supernatant, after removing excess chloroauric acid thus, disperse in 1.5g of pure water again, and prepare gold ion a...

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Abstract

The invention proposes a marker substance and its application in immunology, analyte and test strip. In the measurement of immunology, the resin-metal complex (100) used for labeling substances has resin particles (10) and metal particles (20), and has (A) the average particle diameter of the resin-metal complex exceeds 300 nm, or (B) the average particle diameter of the metal particles is within the range of more than 20 nm and less than 70 nm. Preferably, a part of the metal particles (20) is two-dimensionally or three-dimensionally distributed in the surface part (60) of the resin particle (10), and a part of the three-dimensionally distributed metal particles (20) faces toward the resin particle (10) The outside part is exposed, and the remaining part is enclosed in the resin particle (10). The marking substance is excellent in durability and visibility.

Description

technical field [0001] The present invention relates to an immunological measurement, a marker substance excellent in sensitivity, durability, and visibility, an immunological measurement method using the same, a reagent for immunological measurement, a method for measuring an analyte, and an analyte Measurement kits and test strips for lateral flow chromatography. Background technique [0002] There are countless chemical substances in the living body, so it is an extremely important technology to analyze the specific trace components in the living body qualitatively and quantitatively. In the fields of medicine, pharmaceuticals, health food, biotechnology, environment, etc., drugs and foods that act only on specific parts (chemical substances) in the living body, analysis devices and diagnostic drugs that detect slight changes in the living body, etc. The technologies have evolved together. [0003] One of said analytical techniques is immunoassay. This technique is als...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/553
CPCG01N33/54346G01N33/54388G01N33/553
Inventor 松村康史榎本靖新田龙三
Owner NIPPON STEEL CHEM &MATERIAL CO LTD