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Extraction and purification process of total flavonoids from rose

A technology of rose and total flavonoids, which is applied in pharmaceutical formulations, plant raw materials, plant/algae/fungus/moss components, etc., can solve the problems of low purity and yield, reduce pollution, improve separation and purification efficiency, and achieve reasonable design. Effect

Active Publication Date: 2019-10-18
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem of low purity and low yield in the extraction and purification process of total flavonoids from roses in the prior art, and provides a method that is reasonable, stable, short in production cycle, suitable for industrial production and obtains roses with high purity of total flavonoids Extraction and purification process of total flavonoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The extraction and purification process of rose total flavonoids comprises the following steps:

[0042] A, get the rose dried medicinal material, after pulverizing, add 70% ethanol reflux extraction of 5 times of rose dried medicinal material weight 3 times, each 2 hours, filter the extract, and merge the filtrate;

[0043] B. Remove the ethanol from the extract obtained in step A to obtain a concentrated solution. The concentrated solution is added to the AB~8 macroporous resin, washed with water and 10% ethanol until the water is clear, and the color of ethanol is lighter. Finally, use 80% ethanol was used for elution, and ethanol was removed from the eluent to obtain a secondary concentrated solution;

[0044] C. Pour the secondary concentrated solution obtained in step B into the loaded polyamide column, wash with water and 10% ethanol in turn until the water is clear, the color of ethanol is lighter, and finally elute with 70% ethanol, and the eluent is concentrat...

Embodiment 2

[0049] The difference between this example and Example 1 is that this example also includes treating the rose total flavonoids obtained in step E again according to the step D process, that is, dissolving with methanol, mixing the sample with polyamide, drying the sample, and then The sample is dry-loaded onto the column of column chromatography with polyamide as filler and dichloromethane and methanol as solvents, and the gradient elution is carried out by using dichloromethane:methanol at a ratio of 10:1 to 1:1. , collect the eluate containing total flavonoids according to the TLC plate, and concentrate the eluate to dryness. Wherein, in this step, the amount of polyamide used in the column chromatography is 120 times the total weight of the total crude flavonoids of roses. The volume ratio of dichloromethane and methanol used in the wet column packing is 10:1.

[0050] Determination and calculation, the total flavonoid content in the rose total flavonoid extract of this em...

Embodiment 3

[0052] The extraction and purification process of rose total flavonoids comprises the following steps:

[0053] A, get the rose dried medicinal material, after pulverizing, add 75% ethanol reflux extraction of 10 times of the rose dried medicinal material weight 3 times, each time 2 hours, filter the extract, and merge the filtrate;

[0054] B. Remove ethanol from the extract obtained in step A to obtain a concentrated solution. The concentrated solution is added to the AB~8 macroporous resin, washed with water and 5% ethanol until the water is clear, and the color of ethanol is lighter. Finally, use 85% ethanol was used for elution, and ethanol was removed from the eluent to obtain a secondary concentrated solution;

[0055] C. Pour the secondary concentrated solution obtained in step B into the installed polyamide column, wash with water and 5% ethanol in turn until the water is clear, the color of ethanol is lighter, and finally elute with 75% ethanol, and the eluent is con...

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Abstract

The invention provides a process for extracting and purifying total flavone from rose flower. The total flavone provided by the invention is obtained by extracting dry petals of plant rose flower by using a solvent, and purifying by using macroporous resin, polyamide and C18; the total flavone uses flavonoid compounds as main components. The invention provides a simple, convenient and easily-implemented method for determining the content of the total flavone in the rose flower, and optimizes the process for extracting and purifying the total flavone from the rose flower; the content of the final total flavone from the rose flower is obviously increased, so that the process is wide in application prospect.

Description

technical field [0001] The invention relates to the technical field of extracting total flavonoids from traditional Chinese medicine plants, in particular to a process for extracting and purifying total flavonoids from roses. Background technique [0002] Rose is the dried flower bud of rose (Rosarugosa Thunb), a plant of the family Rosaceae. Roses contain volatile oils, amino acids, polysaccharides and a variety of flavonoids, among which flavonoids have various pharmacological activities such as anti-cancer, immune regulation, hypoglycemic, and anti-oxidation. However, at present, the technological route for providing total flavonoids from roses is immature, mainly due to the fact that the total amount of active ingredients extracted is not high (that is, the purity is low), the technological route is not reasonable enough, or the conditions for industrialization are not available. For example, the publication number is CN102796066A, the name is a kind of flavonoid compou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/738
Inventor 李启发白兰辉李旺赖小红刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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