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Method for separating and extracting berbamine and berberine from root of chinese barberry

A technology for berberamine and berberine, which is applied in the field of separation and extraction of berberamine and berberine, can solve the problems of low extraction rate, waste of raw materials, low separation degree of berberamine and berberine, etc., and achieves high purity , the effect of simple operation

Inactive Publication Date: 2017-05-17
张发民
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages of low extraction rate of conventional technology, low product purity, low separation degree of berbamine and berberine, resulting in waste of raw materials, and provide a kind of salt water solubility characteristic of utilizing quaternary ammonium base and tertiary amine base. The method for separating and extracting berbamine and berberine from three needles, in order to achieve the above object, the principle of the technical scheme adopted by the present invention and the specific steps are as follows

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Isolation of berbamine and berberine from three needles

[0020] ①Take 100g of the coarse powder of three needles, put it in a 1000ml conical flask, add 100ml of 0.2% (volume fraction) dilute sulfuric acid to soak for 24 hours, filter it with gauze, and extract once with 400ml of acid water in the same way, combine the two filtrates and place In an enamel jar (no metal utensils)

[0021] ②Add milk of lime to PH12 under stirring, stand still for 30min, and suction filter, add sodium chloride with a calculated amount of 8% (mass volume fraction) to the filtrate, let stand, let cool to room temperature, and wait for the precipitation to be complete;

[0022] ③ Add concentrated hydrochloric acid dropwise to pH 8.0, and place it in a water bath at 80°C for more than 30 minutes. After the precipitation is flocculated, take it out and let it cool, let it stand, and filter under reduced pressure to collect the precipitate.

[0023] ④ The obtained precipitate was dried at 50...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine extraction and relates to a method for separating and extracting berbamine and berberine from root of chinese barberry. The method mainly utilizes properties of tertiary amine alkaloids and quaternary amine alkaloids for separation. Sulfates of berbamine and berberine are easily soluble in water, berberine hydrochloride is difficult to dissolve in water, berbamine hydrochloride can be dissolved in water, free berberine is a quaternary ammonium alkali soluble in the water, and free berbamine is a tertiary amine alkali insoluble in water. Through use of the above characteristics, the method carries out separation. The used reagents are conventional reagents, complex equipment is avoided, operation is simple, separation effects are good and the single product has high purity.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and in particular relates to a method for separating and extracting berbamine and berberine from three needles. Background technique [0002] The three needles belong to the Berberis genus of the family Berberidaceae, also known as "rhubarb", "thorn coptis" and "muhuanglian" among the people. There are 200 species of this genus distributed in my country, most of which are distributed in the northwest and southwest regions. The three needles are the roots of the dicotyledonous plant Berberis chinensis. The roots contain a variety of alkaloids, including quaternary amines. Alkaloids, such as Berberine (Berberine) 1.2 ~ 3%, root bark contains 3 ~ 5.5%, a small amount of Bama, jatrorrhizine, African tetrandrine, magnolia, tertiary amine bases, such as Berbamine 0.45- 3.84%, spikine, tetrandrine, etc. Its content varies greatly depending on the variety, part, place of o...

Claims

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Application Information

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IPC IPC(8): C07D455/03C07D491/18
CPCC07D455/03C07D491/18
Inventor 张发民
Owner 张发民
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