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Method for separating homozygotes from ploidy chimera plant

A chimera and homozygote technology, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problem of easy generation of ploidy chimeras, difficulty in establishing large tetraploid groups, low tetraploid induction efficiency, etc. problems, to achieve the effect of short cycle, less workload and high efficiency

Active Publication Date: 2017-05-24
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for isolating homozygotes from ploidy chimera plants in view of the low efficiency of tetraploid induction in forest trees, the doubling of somatic cell chromosomes is easy to produce ploidy chimeras, and it is difficult to establish a large tetraploid population. method

Method used

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  • Method for separating homozygotes from ploidy chimera plant
  • Method for separating homozygotes from ploidy chimera plant
  • Method for separating homozygotes from ploidy chimera plant

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Embodiment 1

[0030] The test material of the specific embodiment of the present invention selects the female plant of Populus minor and the male plant of Populus chinensis, and respectively collects flower branches and hydrocultures them in the greenhouse to promote flowering.

[0031] 1. Control pollination hybridization

[0032] Collect the pollen of the male plant Populus chinensis in the greenhouse, and when the pistil of the female plant Populus chinensis enters the fertilization period, dip the pollen with a brush for pollination. During the whole process, attention should be paid to bagging and isolating the pistils to prevent contamination from exogenous pollen. After the pistil stigma wilts, the bagging is removed to allow the female inflorescence to continue to develop normally.

[0033] 2. Seed doubling

[0034] 37, 40, and 43 days after pollination, collect mature female inflorescences without flying catkins, soak them in 75% alcohol for 30 seconds in an ultra-clean workbench...

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Abstract

The invention provides a method for separating homozygotes from a ploidy chimera plant and belongs to the technical field of ploidy breeding of plants. According to the method, leaves of the ploidy chimera plant are subjected to adventitious bud induction by adopting an adventitious bud induction medium, adventitious buds regrown by leaves of the same individual plant are subjected to ploidy detection by a flow cytometry, adventitious buds of different-ploidy homozygotes are obtained through screening and separating and are subjected to regeneration culture, and homozygote plants with different ploidy are obtained. According to the method, the problem about purification of chimeras obtained through somatic chromosome doubling of ligneous plants is solved, an idioblast type plant community of the homozygotes with full-sibling or hetero-sibling diploid, tetraploid or other ploidy can be constructed rapidly and efficiently, and foundation is laid for carrying out polyploid breeding and ploidy effect genetic analysis.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for isolating homozygotes from ploidy chimera plants and establishing full-sib or heterosib cell-type populations. Background technique [0002] Polyploid breeding is an important means of plant genetic improvement, especially for cross-pollinated woody plants, polyploid breeding combines ploidy and heterozygosity, and the improvement effect is remarkable (Kang Xiangyang. About poplar polyploidy Several understandings of sports species. Journal of Beijing Forestry University, 2010,32(5):149-153). Polyploid varieties such as triploid poplar tomentosa and tetraploid black locust have shown significant advantages in terms of growth, wood quality, and metabolite content, and have been widely used in forestry production in my country. [0003] Tetraploid plants are usually obtained by applying treatments to meristem regions such as seeds and shoot tips to induce chrom...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/00A01H4/001
Inventor 王君宋少宇李代丽
Owner BEIJING FORESTRY UNIVERSITY
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