Adjuvant for animal vaccine and its preparation method and application

Abstract

The invention discloses an adjuvant for animal vaccines, and a preparation method and application thereof. The adjuvant for animal vaccines comprises the following raw materials by volume: 75 to 90% of low-density mineral oil, 1 to 10% of octyl and decyl glycerate and Span-80, 0.5 to 5% of glycerin and polyglycerol isostearate and 1 to 10% of polyethylene glycol-12-hydroxystearate-15, Tween-80 and propylene glycol, wherein the amount of Tween-80 is in a range of 0 to 5%. The preparation method comprises the following steps: weighing the low-density mineral oil; then adding octyl and decyl glycerate, Span-80, glycerin, polyglycerol isostearate, 1 to 10% of polyethylene glycol-12-hydroxystearate-15, Tween-80 and propylene glycol; and carrying out mixing and standing. The adjuvant provided by the invention has safety, stability and self-emulsifying performance, has low sensitivity to temperature in the process of emulsification, can form a water-in-oil-in-water spatial structure, and is extensively applicable to preparation of animal vaccines.

Description

technical field [0001] The invention belongs to the field of biological product animal vaccines, and in particular relates to an adjuvant for animal vaccines and a preparation method and application thereof. Background technique [0002] Currently, the two basic components of most animal vaccines are antigens and adjuvants. Adjuvants are like capsules of drugs, which can protect the vaccine and make the vaccine release slowly. Self-emulsification, two-step emulsification and multi-step emulsification will determine the difficulty of the vaccine production process and affect the biological activity of the vaccine. Water-in-oil type (W / O), oil-in-water type (O / W) and water-in-oil-in-water type (W / O / W) will determine the speed of animal immune response and the length of vaccine protection time, among which "water "Refers to the antigen, "oil" refers to the adjuvant, different types refer to the difference in the spatial structure of the oil and water package, and the water-in...

AI Technical Summary

Problems solved by technology

Secondly, the water-in-oil-in-water adjuvant and the antigen preparation process are also unstable. For biological safety considerations, the emulsifier used in the adjuvant is non-ionic, and the HLB value (hydrophilic-lipophilic balance value) of the emulsifier It is one of the keys to form the water-in-oil-in-water type. The HLB value of the non-ionic emulsifier will change with the temperature. If the temperature is not suitable, the water-in-oil-in-water type will be unstable and will become water-in-oil or water-in-oil. Oil-in-oil type, and at the same time, temperature will also affect the biological activity of the antigen, so temperature has become a difficult point in the production process and production equipment of water-in-oil-in-water vaccines. Selecting a suitable emulsifier can reduce the effect of emulsifier HLB value on temperature The sensitivity of the emulsifier is a shortcut to solve the problem, and the existing water-in-oil-in-water does not propose a solution to reduce the sensitivity of the emulsifier HLB value to temperature

[0005] 3. Ease of preparation: Two or more steps of emulsification for the preparation of water-in-oil-in-water adjuvant and antigen will bring cumbersome emulsification process and increase the emulsification process time, which will affect the biological activity of the vaccine

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