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Method for extracting chlorophylls from watermelon peel

A chlorophyll and epidermis technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of low chlorophyll extraction efficiency, chlorophyll damage, and large solvent loss, and achieve uniform extraction effect, thorough extraction, and extraction time. short effect

Inactive Publication Date: 2017-05-24
CHONGQING DOUHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chlorophyll is a fat-soluble substance, which is unstable in heat and decomposes when exposed to light. The heating extraction method takes a long time, and the solvent loss is large, and heating is easy to damage chlorophyll, and the extraction efficiency of chlorophyll is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) The watermelon skin was crushed and beaten, and freeze-dried to obtain a watermelon skin freeze-dried powder. Weigh 100 grams of the watermelon skin freeze-dried powder and set aside.

[0030] 2) Add 95wt% ethanol (freeze-dried watermelon skin: 95wt% ethanol = 1g: 5mL), pretreat at room temperature at 10,000 rpm for 5 minutes at high speed, then extract homogeneously at 3,000 rpm for 30 minutes and filter .

[0031] 3) Collect the filtrate, add 95wt% ethanol to the filter residue (filter residue: 95wt% ethanol = 1g: 5mL), extract homogeneously at room temperature at 3000 rpm for 30 minutes and then filter.

[0032] 4) Combine the two filtrates, and centrifuge at 1000 rpm for 10 minutes at room temperature to remove the precipitate. After collecting the supernatant, vacuum concentrate to obtain a concentrated solution. The solid content in the concentrated solution reaches 30%.

[0033] (5) Stir the concentrated solution with 4 times concentrated solution of acetone ...

Embodiment 2

[0035] 1) The watermelon skin was crushed and beaten, and freeze-dried to obtain a watermelon skin freeze-dried powder. Weigh 100 grams of the watermelon skin freeze-dried powder and set aside. .

[0036] 2) Add 95wt% ethanol (freeze-dried powder of watermelon skin: 95wt% ethanol = 1g: 7mL), pretreat at room temperature at 10,000 rpm for 10 minutes at high speed, then extract homogeneously at 3,000 rpm for 20 minutes and filter .

[0037] 3) Collect the filtrate, add 95wt% ethanol to the filter residue (filter residue: 95wt% ethanol = 1g: 7mL), perform homogeneous extraction at room temperature at 3000 rpm for 20 minutes and then filter.

[0038] 4) Combine the two filtrates, centrifuge at 1000 rpm for 15 minutes at room temperature to remove the precipitate, collect the supernatant and concentrate in vacuo, the solid content in the concentrate reaches 35%.

[0039] 5) Stir the concentrated solution with 8 times concentrated solution of acetone with a volume concentration of...

Embodiment 3

[0041] 1) The watermelon skin was crushed and beaten, and freeze-dried to obtain a watermelon skin freeze-dried powder. Weigh 100 grams of the watermelon skin freeze-dried powder and set aside.

[0042] 2) Add 95wt% ethanol (freeze-dried watermelon skin: 95wt% ethanol = 1g: 10mL), pretreat at room temperature at 10,000 rpm for 10 minutes, then extract homogeneously at 3,000 rpm for 10 minutes and filter .

[0043] 3) Collect the filtrate, add 95wt% ethanol to the filter residue (filter residue: 95wt% ethanol = 1g: 10mL), extract homogeneously at room temperature at 3000 rpm for 10 minutes and then filter.

[0044] 4) Combine the two filtrates, centrifuge at 1000 rpm for 15 minutes at room temperature to remove the precipitate, collect the supernatant and concentrate in vacuo, the solid content in the concentrate reaches 40%.

[0045] 5) Stir the concentrated solution with acetone with a volume concentration of 6 times of 85%, place it at 0-4°C for 0.5 days, filter to remove t...

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PUM

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Abstract

The invention discloses a method for extracting chlorophylls from watermelon peel. The method comprises the following steps: 1, crushing and beating watermelon peel, and freezing obtained slurry to obtain freeze-dried watermelon peel powder; 2, mixing the freeze-dried watermelon peel powder with 95 wt% ethanol, carrying out high-speed pretreatment, carrying out homogenous extraction, and filtering obtained extract; 3, mixing filter residues with 95 wt% ethanol, carrying out homogenous extraction, and filtering obtained extract; 4, centrifuging the obtained filtrate, collecting the obtained supernatant, and concentrating the collected supernatant; and 5, purifying and drying the obtained concentrate to obtain the chlorophylls. The method has the advantages of no heating, less chlorophyll loss, short extraction time, less solvent loss and high extraction efficiency.

Description

technical field [0001] The invention belongs to the technical field of extraction of natural pigments, and in particular relates to a method for extracting chlorophyll from watermelon epidermis. Background technique [0002] my country's watermelon planting technology has developed rapidly in recent years, but most of them are mainly for selling watermelons, the added value of products is low, and the economic benefits are not obvious. The outermost hard skin of watermelon contains a large amount of natural chlorophyll, which can be widely used in food, medicine and other industries; chlorophyll derivatives have the functions of promoting wound healing, anti-ulcer, anti-tumor, anti-microbial, anti-cancer, liver protection, Anti-anemia and many other biological activities. [0003] The skin of watermelon is rich in chlorophyll and is a good source of chlorophyll. [0004] At present, the main extraction method of chlorophyll is heating extraction with solvent method. Chlor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22C09B61/00
CPCC07D487/22C09B61/00
Inventor 曹秀玲其他发明人请求不公开姓名
Owner CHONGQING DOUHAO BIOTECH
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