A Zhongshengmycin fermentation continuous feed production device and production method thereof
A production device and the technology of Shengmycin, which is applied in the field of Zhongshengmycin fermentation continuous feed production equipment, can solve the problems of the destruction of nutrients in the feeding medium, the impact on the fermentation level of Zhongshengmycin, and the high labor intensity of employees. Achieve the effects of prolonging the time of secondary metabolism, good economic and social benefits, and saving manpower and material resources
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Embodiment 1
[0057] The 30L fermenter is used for fermentation and culture of Zhongshengmycin. The continuous feeding device of the fermentor is disinfected and sterilized at the same time as the medium in the fermentor. The continuous feeding device of the fermentor during the fermentation and culture of Zhongshengmycin In a state of aseptic holding pressure. Zhongshengmycin was fermented and cultured for 15 hours, and the reducing sugar content in the fermentation broth was determined to be 2.32%. Started to add 15.00% glucose, 0.50% sodium chloride, 0.55% ammonium chloride, and 0.05% magnesium sulfate. A feed medium containing 0.01% potassium dihydrogen phosphate. Control the feed medium flow to ≤2.50L / h, measure the reducing sugar content of Zhongshengmycin fermentation broth every 1 hour, control the reducing sugar content in the fermentation broth to 1.50%~2.00%, and continue feeding until the end of the fermentation Feeding was stopped for 10 hours, the fermentation broth continued ...
Embodiment 2
[0059] The 30L fermenter is used for fermentation and culture of Zhongshengmycin. The continuous feeding device of the fermentor is disinfected and sterilized at the same time as the medium in the fermentor. The continuous feeding device of the fermentor during the fermentation and culture of Zhongshengmycin In a state of aseptic holding pressure. Zhongshengmycin was fermented and cultured for 25 hours, and the reducing sugar content in the fermentation broth was determined to be 1.96%. Started to add 20.00% glucose, 0.55% sodium chloride, 0.65% ammonium chloride, and 0.04% magnesium sulfate. A feed medium containing 0.01% potassium dihydrogen phosphate, 0.03% glutamic acid, and 0.02% arginine. Control the feed medium flow to ≤2.50L / h, measure the reducing sugar content of the Zhongshengmycin fermentation broth every 1 hour, control the reducing sugar content in the Zhongshengmycin fermentation broth to 1.30%~1.80%, and continue feeding until Feeding was stopped 5 hours before...
Embodiment 3
[0061] The 30L fermenter is used for fermentation and culture of Zhongshengmycin. The continuous feeding device of the fermentor is disinfected and sterilized at the same time as the medium in the fermentor. The continuous feeding device of the fermentor during the fermentation and culture of Zhongshengmycin In a state of aseptic holding pressure. Zhongshengmycin was fermented and cultured for 18 hours, and the reducing sugar content in the fermentation broth was determined to be 2.15%. Started to add 35.00% glucose, 0.45% sodium chloride, 0.55% ammonium chloride, and 0.03% magnesium sulfate. A feed medium containing 0.02% potassium dihydrogen phosphate, 0.05% glutamic acid, and 0.04% arginine. Control the feed flow rate to ≤3.00L / h, measure the reducing sugar content of the Zhongshengmycin fermentation broth every 1 hour, control the reducing sugar content in the Zhongshengmycin fermentation broth at 2.00%~2.50%, and continuously feed for 30 hours; Continue to ferment until t...
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