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A Zhongshengmycin fermentation continuous feed production device and production method thereof

A production device and the technology of Shengmycin, which is applied in the field of Zhongshengmycin fermentation continuous feed production equipment, can solve the problems of the destruction of nutrients in the feeding medium, the impact on the fermentation level of Zhongshengmycin, and the high labor intensity of employees. Achieve the effects of prolonging the time of secondary metabolism, good economic and social benefits, and saving manpower and material resources

Active Publication Date: 2019-04-09
福建凯立生物制品有限公司 +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. The current feed medium adopts the well-known damp heat sterilization method in the industry. Since the damp heat sterilization needs to reach 121°C and the sterilization maintenance time is 30 minutes, the high temperature process of the feed medium above 80°C takes as long as 60 Minutes or more, causing the appearance of the feed medium to turn red or brown after a long period of high temperature, the nutrients in the feed medium are destroyed, and even produce substances that are toxic to the Zhongshengmycin-producing bacteria, affecting the Zhongshengmycin Increased fermentation level
[0006] 2. The current feeding process is intermittent large-scale feeding, which causes large fluctuations in the composition of the medium in the fermenter, which has a great impact on the micro-growth environment of the Zhongshengmycin-producing bacteria, which is not conducive to the growth and secondary production of the producing bacteria. growth of metabolites
[0007] 3. The current fermentation process intermittently feeds a large amount of materials, which is easy to form the well-known glucose effect in the industry, resulting in the inhibition of the formation of secondary metabolites and affecting the growth of Zhongshengmycin production
[0008] 4. The current intermittent large-scale feed makes the nutrition excess in the early and mid-term of fermentation, and a large number of bacterial cells multiply; the nutrition in the middle and late stages is insufficient, which cannot further improve the fermentation level of Zhongshengmycin, and the content of Zhongshengmycin in the fermentation liquid hovers at 8.00g / L— Between 10.00g / L
[0009] 5. The current intermittent large-scale feeding process needs to consume a large amount of steam for multiple feeding medium disinfection and multiple feeding pipeline disinfection, which results in high labor intensity for employees and a large amount of energy consumption

Method used

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  • A Zhongshengmycin fermentation continuous feed production device and production method thereof

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Experimental program
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Effect test

Embodiment 1

[0057] The 30L fermenter is used for fermentation and culture of Zhongshengmycin. The continuous feeding device of the fermentor is disinfected and sterilized at the same time as the medium in the fermentor. The continuous feeding device of the fermentor during the fermentation and culture of Zhongshengmycin In a state of aseptic holding pressure. Zhongshengmycin was fermented and cultured for 15 hours, and the reducing sugar content in the fermentation broth was determined to be 2.32%. Started to add 15.00% glucose, 0.50% sodium chloride, 0.55% ammonium chloride, and 0.05% magnesium sulfate. A feed medium containing 0.01% potassium dihydrogen phosphate. Control the feed medium flow to ≤2.50L / h, measure the reducing sugar content of Zhongshengmycin fermentation broth every 1 hour, control the reducing sugar content in the fermentation broth to 1.50%~2.00%, and continue feeding until the end of the fermentation Feeding was stopped for 10 hours, the fermentation broth continued ...

Embodiment 2

[0059] The 30L fermenter is used for fermentation and culture of Zhongshengmycin. The continuous feeding device of the fermentor is disinfected and sterilized at the same time as the medium in the fermentor. The continuous feeding device of the fermentor during the fermentation and culture of Zhongshengmycin In a state of aseptic holding pressure. Zhongshengmycin was fermented and cultured for 25 hours, and the reducing sugar content in the fermentation broth was determined to be 1.96%. Started to add 20.00% glucose, 0.55% sodium chloride, 0.65% ammonium chloride, and 0.04% magnesium sulfate. A feed medium containing 0.01% potassium dihydrogen phosphate, 0.03% glutamic acid, and 0.02% arginine. Control the feed medium flow to ≤2.50L / h, measure the reducing sugar content of the Zhongshengmycin fermentation broth every 1 hour, control the reducing sugar content in the Zhongshengmycin fermentation broth to 1.30%~1.80%, and continue feeding until Feeding was stopped 5 hours before...

Embodiment 3

[0061] The 30L fermenter is used for fermentation and culture of Zhongshengmycin. The continuous feeding device of the fermentor is disinfected and sterilized at the same time as the medium in the fermentor. The continuous feeding device of the fermentor during the fermentation and culture of Zhongshengmycin In a state of aseptic holding pressure. Zhongshengmycin was fermented and cultured for 18 hours, and the reducing sugar content in the fermentation broth was determined to be 2.15%. Started to add 35.00% glucose, 0.45% sodium chloride, 0.55% ammonium chloride, and 0.03% magnesium sulfate. A feed medium containing 0.02% potassium dihydrogen phosphate, 0.05% glutamic acid, and 0.04% arginine. Control the feed flow rate to ≤3.00L / h, measure the reducing sugar content of the Zhongshengmycin fermentation broth every 1 hour, control the reducing sugar content in the Zhongshengmycin fermentation broth at 2.00%~2.50%, and continuously feed for 30 hours; Continue to ferment until t...

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Abstract

The invention discloses a zhongshengmycin fermentation continuous feeding production device and a production method thereof. A feeding medium in a zhongshengmycin fermentation process is not subjected to steam high-temperature moist heat disinfection and sterilization; the feeding medium is equipped with a conveying system, a flow meter for measurement and a sterilization filtering system to obtain a sterile feeding medium which is continuously added into a fermentation tank, so that zhongshengmycin fermentation is continuously and normally performed; and the sterilization filtering system realizes disinfection and sterilization and pressure maintaining at the time of the disinfection and sterilization of a fermentation medium in the fermentation tank. In the invention, the labor intensity of workers is greatly relieved, the feeding control in the zhongshengmycin fermentation process is more flexible and accurate, the steam is remarkably saved, and the fermentation production level of zhongshengmycin is improved. The zhongshengmycin fermentation continuous feeding production device and production method thereof disclosed by the invention are not reported yet.

Description

Technical field [0001] The invention relates to the fermentation production of biological pesticides, in particular to a zhongshengmycin fermentation continuous feed production device and a production method thereof. Background technique [0002] According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), in the process of agricultural and forestry production in the world, the annual losses caused by diseases, insects and weeds are more than one-third. Approximately 3 million chemical pesticide poisoning incidents occur worldwide every year. In developing countries, there are approximately 1,650 collective poisoning incidents caused by the consumption of pesticides contaminated food every year. Therefore, it has become an inevitable trend and a global consensus to vigorously develop safe, low- and non-toxic, low-accumulation and no-accumulation biological pesticides that do not pollute the environment. Zhongshengmycin is a new type of agric...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12P19/28
CPCC12M29/04C12P19/28
Inventor 陈俊升陈灼湖温锦奋刘炳珠朱建伟赵立平
Owner 福建凯立生物制品有限公司