Streptomyces capoamus with capacity of killing root-knot nematodes and developed biological breeding substrate thereof

A seedling-raising matrix and seedling-raising biological matrix technology are applied to the field of Streptomyces endophytes in bananas and the biological seedling-raising matrix developed by them, which can solve the problems of adverse effects, short duration of chemical nematicide, etc., so as to promote growth and enhance prevention and control. The effect of root-knot nematodes and the effect of promoting growth

Active Publication Date: 2017-05-24
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in domestic production, the substrate and the soil are mostly isolated, and chemical nematicides are used to prevent and control root-knot nematodes during seedling cultivation. However, the duration of chemical nematicides is short, and multiple applications are likely to have adverse effects on bananas and soil. Therefore, this patent research has become more important

Method used

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  • Streptomyces capoamus with capacity of killing root-knot nematodes and developed biological breeding substrate thereof
  • Streptomyces capoamus with capacity of killing root-knot nematodes and developed biological breeding substrate thereof
  • Streptomyces capoamus with capacity of killing root-knot nematodes and developed biological breeding substrate thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation and identification of strains

[0034] Wash the banana roots and cut them into small sections of about 3cm. Weigh 10g of roots, sterilize with 5% NaClO for 8 minutes, wash 3 times with sterile water, sterilize with 75% ethanol for 5 minutes, wash 3 times with sterile water, and place them in 10ml sterile water Grind in a mortar and apply a gradient dilution of the grinding solution. After culturing in the dark for 3-4 d in an incubator, a single colony was picked, and after plate purification, a total of 150 strains were selected, and the inhibitory effects of the fermentation broth of each strain on root knot nematodes were determined. A strain NAU-SA-1 with excellent sterilizing effect was obtained and kept for further study.

[0035] The colony of strain NAU-SA-1 on Gao's No. 1 plate is light yellow, dry and opaque, and the surface is dense and velvety. The connection between the colony and the culture medium is tight, the edges are not neat, and it i...

Embodiment 2

[0037] Example 2 Production of functional bacteria liquid

[0038] The strain NAU-SA-1 (CGMCC NO.10441) was inoculated into Gao's No. 1 culture broth for liquid fermentation production. The conditions for fermentation production are: pH 7.2~7.4, fermentation temperature range 30°C, stirring speed 170 rpm , Spores are formed in the middle and late stages of fermentation, and the fermentation time is 7 days, so that the amount of bacteria or spores in the fermentation broth is ≥3×10 7 Pcs / ml; the preparation method of the Gao's No. 1 medium used is, taking the preparation of 1L medium as an example: soluble starch 20g, K 2 HPO 4 0.5g, KNO 3 1g, MgSO 4 0.5g, FeSO 4 0.01g, dilute to 1000ml, pH 7.2~7.4, sterilize at 121℃ for 20min.

Embodiment 3

[0039] Example 3 Colonization effect of functional microorganisms in banana roots

[0040] Transfer the rooting sterile bagged banana seedlings to the tissue culture bottle containing the rooting medium, all operations are aseptic conditions, inoculate 5% of the strain NAU-SA-1 bacterial solution in the banana root cell culture medium. The colonization statistics of NAU-SA-1 use surface disinfection and coating counting: banana roots are washed and weighed, 5% NaClO disinfection for 8 minutes, sterile water washing 3 times, 75% ethanol disinfection for 5 minutes, sterile water washing 3 times , Grind the root samples in a sterile mortar and apply the grinding solution. The preparation method of Gao's No. 1 medium is to prepare 1L medium as an example: soluble starch 20g, K 2 HPO 4 0.5g, KNO 3 1g, MgSO 4 0.5g, FeSO 4 0.01g, 20-30g agar powder, dilute to 1000ml, pH 7.2-7.4, sterilize at 121℃ for 20min. The number of functional bacteria NAU-SA-1 strains is calculated by root we...

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Abstract

The invention provides streptomyces capoamus with capacity of killing root-knot nematodes and a developed biological breeding substrate thereof. The streptomyces capoamus NAU-SA-1 is collected in China General Microbiological Culture Collection Center, is collected on January 26, 2015 and has a collection number of CGMCC NO. 10441. A functional biological vegetable breeding substrate is obtained by adding the bacterium liquid of the streptomyces capoamus NAU-SA-1 disclosed by the invention into an ordinary breeding substrate. The product selects specific endophytic actinomycetes for banana seedlings, and is kept alive to be added into the substrate so as to obtain the active biological substrate product. The product has an excellent effect of promoting growth of bananas in the seedling period, is capable of breeding high-quality seedlings with lots of active functional microbes in roots, and has the effect of enhancing the resistance capacity of the bred seedlings on the root-knot nematodes.

Description

Technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a banana endophytic streptomycete with the ability to kill root-knot nematodes and a biological seedling raising substrate developed by the strain. Background technique [0002] Banana is a large herbaceous fruit tree unique to tropical and subtropical regions. Meloidogyne spp. is a colonizing endoparasite of plant roots. It is one of the main pathogenic nematodes of crops in my country. It often causes crop yield losses of 10% to 30%, and in severe cases, it can reach more than 70%. The important economic crops cause losses of up to tens of billions of dollars each year. It is distributed in most provinces and regions in my country and can infect hundreds of crops, among which vegetable and fruit trees suffer the most. In the production process, secondary seedling production technology is particularly important. Generally speaking, the process of banana pseudo-planting has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G9/10A01N63/02A01P21/00A01P5/00C12R1/465
CPCA01G24/00A01N63/10C12N1/205C12R2001/465
Inventor 沈其荣李荣苏兰茜沈宗专张瑞福
Owner NANJING AGRICULTURAL UNIVERSITY
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