Regular morphology of fusarium oxysporum laboratory material and preparation and application method thereof

The invention discloses a laboratory technology of banana fusarium wilt, which is applied to the standing form of laboratory materials of banana fusarium wilt and its preparation and application method. It can solve the problems of not being able to carry out in time and delay, and achieve the effects of standardization, convenient and fast work, and accurate and quantitative access

Inactive Publication Date: 2017-05-24
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This means that the relevant work process needs

Method used

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Embodiment 1

[0037] Apply the present invention's standing form of laboratory materials of Fusarium wilt of banana and its preparation and application method, cultivate and prepare the laboratory standing form material of Fusarium wilt of banana bacterial strain Fo-1 with conidia as the thallus; preparation and application of bacterial strain Fo-1 The standing form of the material is operated according to the following steps:

[0038] 1. Preparation and culture of conidia

[0039] Use a triangular flask as a culture device, and fill the potato glucose medium as a spore-producing medium. The composition ratio of the medium is: 200g of potatoes, 20g of glucose, 20g of agar, and 1000mL of water; The filaments were transplanted into the spore-forming medium in the Erlenmeyer flask, and after culturing at 28° C. for 5 days, the colonies on the surface of the medium formed a large number of conidia.

[0040] 2. Preparation of standing form of banana Fusarium wilt material

[0041]Inject steril...

Embodiment 2

[0051] Apply the present invention's standing form of laboratory materials of Fusarium wilt of banana and its preparation and application method, cultivate and prepare the laboratory standing form material of Fusarium wilt of banana strain Fo-2 with conidia as thallus; preparation and application of bacterial strain Fo-2 The standing form of the material is carried out according to step 1 to step 5 of embodiment 1, the difference is that the bacterial strain used in step 1 is Fo-2, and step 2 obtains the standing spore liquid of Fusarium wilt bacterial strain Fo-2.

[0052] Use a pipette to quantitatively draw 5 μL of the calibrated standing spore solution, transplant it to the middle of the potato glucose culture plate, and culture it at 28°C for 2 days, and it can grow into a typical small colony.

[0053] Take 10 μL of the calibrated standing spore spore liquid, transplant it into the potato glucose culture medium, shake it at 28°C for 5 days, and form a large number of new ...

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Abstract

The invention discloses a regular morphology of a fusarium oxysporum laboratory material and a preparation and application method thereof. Fusarium oxysporum pure and pollution-free conidiospore is used as a regular morphology of the laboratory material. The conidiospore serving as the regular morphology is stored in sterile water. The preparation and application method comprises the following steps that 1, a triangular flask serves as a culture container to prepare the fusarium oxysporum conidiospore; 2, the regular morphology of the fusarium oxysporum material is prepared; 3, the conidiospore content of the regular morphology of the fusarium oxysporum material is calibrated; 4, the regular morphology of the fusarium oxysporum material is daily stored; 5, the regular morphology of the fusarium oxysporum material is applied. The regular morphology has the characteristics and advantages that the conidiospore serving as the regular morphology can suspend to grow in pure water and can be instantly restored to normal growth when being transferred to a culture medium; the regular morphology can be applied in relevant work at any time, a thallus material can be directly provided for a test link needing the conidiospore, the working process needing to repeatedly prepare and culture the conidiospore when a mycelium is used as a commonly prepared material can be omitted, and work is convenient and quick; 3, the triangular flask serves as the culture container, and pure and pollution-free conidiospore liquid is easy to prepare; the conidiospore content of the commonly prepared conidiospore liquid can be calibrated and can be accurately quantified and taken in application, and standardization of a technical method is promoted.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a standing form of a laboratory material of Fusarium wilt of banana and a preparation and application method thereof. Background technique [0002] Banana Fusarium wilt is a major disease on bananas, which can cause devastating losses to banana production. The disease is caused by the infection of Fusarium oxysporum. Banana Fusarium wilt is easy to grow on ordinary culture medium, and mycelium is well developed; in laboratory work, banana Fusarium wilt is usually cultivated into slant colonies with test tube slant medium, and the mycelium material of the colonies is used as a standing stock in daily work Material. The starting point of the relevant research work is the mycelium material as the starting point, and through the transplantation of the standing material mycelium, the expanded cultivation or the research work of specific projects are carried out. This kind ...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/77
Inventor 王忠文张君成李界秋
Owner GUANGXI UNIV
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