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Method for improving output of nikkomycin

A technology of nikkomycin and inoculum amount, which is applied in the field of microorganisms, can solve the problems of large consumption of raw materials and low yield, and achieve the effects of increased yield, low cost, and good genetic stability

Active Publication Date: 2017-05-24
牡丹江佰佳信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the method for preparing nikkomycin in the prior art has the problems of low yield and large consumption of raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The invention provides a method for preparing nikkomycin by fermenting Streptomyces chromogenes BJX005, comprising the following steps:

[0030] Step (1): Prepare the seed liquid of Streptomyces chromogenes, and inoculate the seed liquid in a fermentation medium with an inoculation amount of 4% to carry out fermentation culture to obtain a fermentation liquid; the medium contains the following: Components: 3g corn starch, 3g soybean meal powder, 0.2g yeast powder, 0.6g sodium chloride, 0.04 ammonium sulfate, 0.5g calcium carbonate, polysiloxane defoamer SXP-101-10.02g, Tween 800.02g , add water to 100g. Heat to 80°C to dissolve, and after cooling, use sodium hydroxide to adjust the pH value to 6.8; the fermentation culture is shaking culture, and the rotation speed of shaking culture is 220r / min, the fermentation time is 70h, and the fermentation temperature is 28°C; the fermentation vessel uses 8 After sealing with a layer of gauze, sterilize at 121°C for 30 minutes; ...

Embodiment 2

[0034] The invention provides a method for preparing nikkomycin by fermenting Streptomyces chromogenes, comprising the following steps:

[0035] Step (1): Prepare the seed liquid of Streptomyces chromogenes, and inoculate the seed liquid in a fermentation medium with an inoculation amount of 10% to carry out fermentation culture to obtain a fermentation liquid; the medium contains the following Components: cornstarch 2.5g, soybean meal powder 2g, yeast powder 0.2g, sodium chloride 0.4g, ammonium sulfate 0.05, calcium carbonate 0.45g, polysiloxane defoamer DF-805 (purchased from Guangdong Dongguan De Feng defoamer factory) 0.025g, Tween 800.01g, add water to 100g. Heat to 80°C to dissolve, and after cooling, use sodium hydroxide to adjust the pH value to 7.5; the fermentation culture is vibration culture, and the rotation speed of vibration culture is 200r / min, the fermentation time is 80h, and the fermentation temperature is 25°C; the fermentation vessel is 10 After sealing w...

Embodiment 3

[0039] Compared with Example 1, the only difference is that the defoamer used in this example is a GPE polyether defoamer, i.e. foam enemy, and other conditions are the same.

[0040] The nikkomycin prepared in this example was tested, and the yield was 40300 μg / mL.

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PUM

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Abstract

The invention provides a method for improving the output of nikkomycin. The invention specifically provides a fermentation medium for Streptomyces ansochromogenes and a method for preparing nikkomycin by using the medium. According to the invention, a proper amount of Tween 80 is added into the fermentation medium to further optimize the varieties and amounts of specific components of the fermentation medium and other culture conditions are controlled, so the output of nikkomycin is increased; the output of nikkomycin in the invention is as high as 42600 [mu]g / mL; and the method is simple, low in cost and suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to the field of microorganisms, and specifically provides a fermentation medium for Streptomyces chromogenes and a method for preparing nikkomycin by using the medium. Background technique [0002] Nikkomycin is an important peptidyl nucleoside antibiotic. It has a good control effect on fungal diseases of crops, fruits and vegetables and some economic plants. It is also environmentally friendly and suitable for large-scale promotion and use in agricultural production. Summary of the traditional preparation methods, the method of mutagenesis and screening of excellent strains is usually used to increase the yield of nikkomycin, but this method has many uncertain factors, large screening work, slow breeding, and it is difficult to effectively increase the nikkomycin yield Shortcomings. [0003] Nicomycin has a similar structure to UDP-N-acetylglucosamine, the natural substrate of chitin synthase, and competitively inhibits chitin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/465
Inventor 周贤龙石怀月刘静
Owner 牡丹江佰佳信生物科技有限公司
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