Rs8099917 genotyping dual-color fluorescence PCR rapid detection kit

A detection kit and two-color fluorescence technology, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problems of difficult general hospitals, expensive equipment, poor specificity, etc., and achieve high sensitivity High, short detection time, easy operation
CN106701918AInactive Publication Date: 2017-05-24WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
WUHAN UNIV
Publication Date
2017-05-24
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses an rs8099917 locus gene based genotyping dual-color fluorescence PCR rapid detection kit. Primers and TaqMan-MGB probes are redesigned to optimize the reaction system, a two-component hot start DNA polymerase composes an enzyme activity automatic regulating system, ROX Reference Dye can eliminate a signal background and correct the fluorescence signal error generated between holes, thus realizing accurate, high amplification efficiency, high sensitivity, good specificity, good repeatability, simple operation and short-time detection. Therefore, the technology can achieve clinical application and promotion.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a rapid detection kit for two-color fluorescence PCR based on rs8099917 site genotyping. Background technique

[0002] Hepatitis C is a viral hepatitis caused by hepatitis C virus (HCV) infection, which is mainly transmitted through blood transfusion, acupuncture and drug abuse. Existing studies have shown that the rs8099917 polymorphism of the human IL-28B gene is correlated with the efficacy of antiviral therapy.

[0003] At present, there are many methods for identifying the polymorphism of the rs8099917 locus gene, mainly using methods such as gene sequencing, gene chip, high-resolution melting curve (HRM) and enzyme digestion, but most of the operations are complicated, time-consuming, and require The equipment is expensive and the specificity is poor, so it is difficult to carry out in ordinary hospitals. Contents of the invention

[0004] The purpose of the present inventi...

Claims

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