Rs12979860 genotyping dual-color fluorescent PCR rapid detection kit

A detection kit and two-color fluorescence technology, applied in the biological field, can solve the problems of difficult deployment in general hospitals, expensive equipment, complicated operation, etc., and achieve the effects of high sensitivity, short detection time, and easy operation

Inactive Publication Date: 2017-02-15
WUHAN UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for identifying the polymorphism of the rs12979860 locus gene, mainly using methods such as gene sequencing, gene chip, high-resolution melting curve (HRM) and enzyme digestion, but most of the operations are complicated, time-consuming, and require The equipment is expensive and the specificity is poor, so it is difficult to carry out in ordinary hospitals

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rs12979860 genotyping dual-color fluorescent PCR rapid detection kit
  • Rs12979860 genotyping dual-color fluorescent PCR rapid detection kit
  • Rs12979860 genotyping dual-color fluorescent PCR rapid detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment provides a rapid detection kit for rs12979860 locus genotyping two-color fluorescent PCR

[0025] 1. Kit composition and preparation

[0026] 1) Primers and probe sets

[0027] Primer probe set based on rs12979860 site

[0028] Forward primer F, the nucleotide sequence of which is shown in SEQ ID NO.1;

[0029] 5'CGGTCGTGCCTGTCGTGT3';

[0030] Reverse primer R, the nucleotide sequence of which is shown in SEQ ID NO.2;

[0031] 5'AGCGCGGAGTGCAATTCA 3';

[0032] C fluorescent probe S1, the nucleotide sequence of which is shown in SEQ ID NO.3;

[0033] 5'FAM-ACCCTGGTTCGCGCC-NFQ 3'-MGB, FAM probe;

[0034] T fluorescent probe S2, the nucleotide sequence of which is shown in SEQ ID NO.4;

[0035] 5'VIC-TGGTTCACGCCTTC-NFQ 3'-MGB, VIC probe;

[0036] The above primers and probes were all synthesized artificially by Shanghai Sangon Biotechnology Co., Ltd.

[0037] 2) DNA extraction solution

[0038] The DNA extraction solution can use a commercially av...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an rs12979860 locus genotyping dual-color fluorescent PCR rapid detection kit. According to the kit, a primer and a TaqMan-MGB probe are redesigned, a reaction system is optimized, bi-component hot-start DNA polymerase forms an enzyme activity automatic regulation system, ROX Reference Dye can eliminate a signal background and correct fluorescence signal errors between holes, therefore, the kit achieves accuracy, high amplification efficiency, high sensitivity, good specificity, good repeatability, easy and convenient operation and shorter detection time, and the technology can be applied and popularized clinically.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid detection kit for two-color fluorescence PCR based on rs12979860 site genotyping. Background technique [0002] Hepatitis C is a viral hepatitis caused by hepatitis C virus (HCV) infection, which is mainly transmitted through blood transfusion, acupuncture and drug abuse. Existing studies have shown that the rs12979860 polymorphism of the human IL-28B gene is correlated with the spontaneous clearance of HCV and the efficacy of antiviral therapy. [0003] At present, there are many methods for identifying the polymorphism of the rs12979860 locus gene, mainly using methods such as gene sequencing, gene chip, high-resolution melting curve (HRM) and enzyme digestion, but most of the operations are complicated, time-consuming, and require The equipment is expensive and the specificity is poor, so it is difficult to carry out in ordinary hospitals. Contents of the invention [...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2531/113C12Q2561/101
Inventor 赵友云孙莉军郑毅王业富
Owner WUHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap