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Efficient and rapid erythrocyte sedimentation agent and preparation method thereof

An erythrocyte sedimentation and rapid technology, used in the preparation of test samples, sedimentation analysis, particle and sedimentation analysis, etc., can solve the problem of inappropriate clinical diagnostic testing or a large number of scientific research activities, separation materials cannot effectively obtain high quality, testing or clinical Diagnose problems such as impact, achieve high clinical and scientific research application value, rapid and safe separation, and simple and easy preparation methods.

Inactive Publication Date: 2017-05-24
NANJING XIANZHUO BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently, there are many methods for separating erythrocytes from blood samples, but some of them often use centrifugation methods, such as US patents 6673629, 4678757, 6818180 and published patent applications 2004 / 0202783, 2006 / 0029923, etc., which mostly use physical or chemical methods Separation of red blood cells in whole blood is easy to produce coagulation samples, and the separation is incomplete, especially in chromatographic immunoassays, which often lead to some non-specific binding or strong background interference, and the technical means are complicated and expensive, which is not suitable for clinical diagnosis detection or substantial research activity
In addition, some separation materials cannot effectively obtain blood samples with high quality, high survival rate and no red blood cells, which often have a great impact on detection or clinical diagnosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Preparation of modified starch:

[0013] 1. Weigh 3g of dried potato starch and dissolve it in 20mL of distilled water, stir it evenly and pour it into a 100mL three-necked bottle. Ferrous chloride tetrahydrate, then add dropwise 14mL 30% hydrogen peroxide;

[0014] 2. After fully stirring and reacting for 5 hours, let the three-necked bottle cool to room temperature, pour the mixture into a 250mL beaker and use absolute ethanol to completely precipitate the white precipitate and wash until the supernatant pH test paper does not change color;

[0015] 3. Stand still and filter the precipitate, dry to obtain powder;

[0016] Preparation of erythrocyte sedimentation agent:

[0017] Take 5g of modified starch and add it to 0.5mol / L lye, fully dissolve it, then add an appropriate amount of sodium chloride to make a solution of 5% modified starch + 0.9% sodium chloride, stir at 25°C for 30min, and press After steam sterilization (121°C, 20min), sodium carboxymethyl starch...

Embodiment 2

[0019] Preparation of modified starch:

[0020] 1. Weigh 6g of dried potato starch and dissolve it in 40mL of distilled water, stir well and pour it into a 100mL three-neck bottle, keep the water bath at a constant temperature of 30°C, stir with a stirrer, then add 5g of maleic acid and 28mg of tetrahydrate respectively Ferrous chloride, then add 8 mL of 30% hydrogen peroxide dropwise;

[0021] 2. After fully stirring and reacting for 6.5 hours, let the three-necked bottle cool to room temperature, pour the mixture into a 250mL beaker and wash with absolute ethanol until the white precipitate is completely precipitated and the pH test paper of the supernatant does not change color;

[0022] 3. Stand still and filter the precipitate, dry to obtain powder;

[0023] Preparation of erythrocyte sedimentation agent:

[0024] Take 6g of modified starch and add it to 0.6mol / L lye, fully dissolve, then add an appropriate amount of sodium chloride to make a solution of 6% modified sta...

Embodiment 3

[0026] Preparation of modified starch:

[0027] 1. Weigh 7g of dried potato starch and dissolve it in 50mL of distilled water, stir well and pour it into a 100mL three-neck bottle, keep the water bath at a constant temperature of 35°C, stir with a stirrer, then add 5g of maleic acid and 30mg of tetrahydrate respectively Ferrous chloride, then add 5mL of 30% hydrogen peroxide dropwise;

[0028] 2. After fully stirring and reacting for 8 hours, let the three-neck flask cool to room temperature, pour the mixture into a 250mL beaker and wash with 95% ethanol until the white precipitate is completely precipitated and the pH test paper of the supernatant does not change color;

[0029] 3. Stand still and filter the precipitate, dry to obtain powder;

[0030] Preparation of erythrocyte sedimentation agent:

[0031] Take 7g of modified starch and add it to 0.75mol / L lye, fully dissolve it, then add an appropriate amount of sodium chloride to make a solution of 7% modified starch + 0...

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Abstract

The invention discloses an efficient and rapid erythrocyte sedimentation agent and a preparation method thereof. The method relates to the field of clinical examination, medical research and other fields using whole blood samples of people. The preparation method comprises the following steps of adopting starch as a raw material, carrying out carboxylation on the raw material under a certain condition to prepare high substituted modified starch, and co-mixing the modified starch and sodium chloride according to a certain proportion to prepare the erythrocyte sedimentation agent. The erythrocyte sedimentation agent can be used for quickly and efficiently separating and sedimentating erythrocyte from whole blood, is high in safety, cheap in raw material which is easy to get, low in production cost, simple, convenient and easy in preparation process, and suitable for industrial production in large scale.

Description

technical field [0001] An efficient and rapid erythrocyte sedimentation agent and a preparation method thereof, the invention relates to the fields of clinical examination and medical research, etc., which use a large number of human whole blood samples. Background technique [0002] Nowadays, with the continuous development of surgical blood transfusion, special disease treatment, clinical testing and scientific research work, blood samples are urgently needed, but the general use is limited to serum or plasma without red blood cells. One of the reasons is that red blood cells are used in many clinical diagnoses. There will be some interference during the detection process, and secondly, in some chromatographic detection devices, especially immunoassays, red blood cells are most likely to be the main factor causing fluid flow obstruction, because fluid flow is critical to the reaction that occurs during the detection Therefore, in most cases, the use of blood samples requir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/05G01N1/40
CPCG01N15/05G01N1/40G01N1/4055
Inventor 尹鸿萍苑仁强乐龙
Owner NANJING XIANZHUO BIOLOGICAL ENG CO LTD
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