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A kind of rapid propagation method of Ilex holly tissue culture

A technique for tissue culture and holly japonica, applied in the field of plant reproduction, can solve the problems of growth and destruction of holly japonica, small seeds of holly japonica, time-consuming and labor-intensive collection, etc., and achieves the effects of shortening seedling raising time, simple operation and easy implementation.

Inactive Publication Date: 2019-06-21
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the seeds of holly holly are small, and its collection is time-consuming and labor-intensive, and it is not easy to store
The cutting propagation of Ilex umeleaf needs to collect a large number of lignified branches of the year, which will cause great damage to the growth of Ilex umeleaf, and it is easy to be limited by the amount of materials in large-scale planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a. Select 8-12cm healthy wild holly holly shoots with new buds; remove the miscellaneous leaves of the branches, use alcohol cotton with a volume fraction of 75% to wipe off the dust on the surface of the branches and cut them into 3-5 sections. The segment is about 3cm, with 1-2 dormant bud points, and the branch stem segment is used as the explant;

[0024] B, explant sterilization: on the aseptic workbench, the stem section (explant) obtained by the above-mentioned preliminary disinfection is placed in a volume fraction of 75% alcohol solution and soaked for 30s; Put the sterilized stem section into an Erlenmeyer flask, add 0.1% mercuric chloride aqueous solution, drop 1 drop of Tween, soak for 10-15min, shake several times in the middle, and finally rinse 3 times with sterile water to obtain sterilized Treated explants;

[0025] c. Induction culture of explants: insert the sterilized explants into the first-generation germination induction medium for culture, the c...

Embodiment 2

[0031] a. Select 8-12cm healthy wild holly holly shoots with new buds; remove the miscellaneous leaves of the branches, use alcohol cotton with a volume fraction of 75% to wipe off the dust on the surface of the branches and cut them into 3-5 sections. The section is about 3cm, with 1-2 dormant bud points, and the branches treated with this are used as explants;

[0032] B, explant sterilization: on the aseptic workbench, the stem section (explant) obtained by the above-mentioned preliminary disinfection is placed in a volume fraction of 75% alcohol solution and soaked for 30s; Put the sterilized stem section into an Erlenmeyer flask, add 0.1% mercuric chloride aqueous solution, drop 1 drop of Tween, soak for 10-15min, shake several times in the middle, and finally rinse 3 times with sterile water to obtain sterilized Treated explants;

[0033] c. Induction culture of explants: insert the sterilized explants into the primary germ-inducing medium for culture, the culture condi...

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PUM

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Abstract

The invention discloses a rapid propagation method for tissue culture of ilex asprella. The rapid propagation method realizes rapid propagation for tissue culture of wild ilex asprella, and opens a new way for a tissue culture technology of the wild ilex asprella. The method provided by the invention is simple to operate and easy to implement, and can cultivate a large number of seedlings of the ilex asprella at one time. The method provided by the invention can effectively shorten the time of seedling raising, and a culture medium and a reagent used in the method are easy to prepare, so that the method is low in cost and easy to popularize on a large scale.

Description

[0001] Technical field: [0002] The invention belongs to the field of plant propagation, and in particular relates to a rapid propagation method for tissue culture of holly holly. [0003] Background technique: [0004] Ilex asprella Champ.ex Benth belongs to Ilex family Ilex, a deciduous shrub with a plant height of up to 3 meters. It is mainly distributed in Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Guangdong, Guangxi, Hong Kong and other places in China; it is also distributed in the Philippine Islands. It mostly grows in mountain forests or roadside thickets at an altitude of 400-1000 meters. Its roots, stems, and leaves can be used as medicine. It tastes bitter, sweet, and cool in nature. It can clear away heat and detoxify, promote body fluid and quench thirst. It is a traditional Chinese medicine commonly used in Lingnan areas such as Guangdong and Guangxi. It is used for colds, high fever and polydipsia, tonsillitis, pharyngitis, tracheitis, whooping cough, etc. Plu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 公绪云蔡锡安周丽霞曾宋君吴坤林刘占锋王晓玲
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI