Application of naphthalenesulfonamide compounds in regulation of plant growth activity
A plant growth and compound technology, applied in the application field of naphthalene sulfonamide compounds in regulating plant growth activity, can solve the problems of high production cost, and achieve the effect of improving cold resistance and excellent plant growth regulating activity
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Embodiment 1
[0017] Example 1. Determination of biological activity of compound improving cold tolerance of rice (Nipponbare, purchased from Rice Group, Agricultural College, China Agricultural University)
[0018] Spray the seedling tray wet, sprinkle a little vermiculite (water-absorbing, moisturizing) in the hole as a bedding, then spray water, sow, pay attention to the root facing down, touching the bottom of the tray, and the bud facing upward, then sprinkle vermiculite to bury the seeds, every 30 seeds were treated with 5 repetitions. Spray with water again to keep hydrated. Sprinkle more vermiculite to prevent premature evaporation of water. After the sowing, the bottom of the tray was covered with shallow water (a nutrient solution with a concentration of 30% by mass, see Table 3 for specific components). Spray the seedling trays with water daily to keep them hydrated. The seedling trays were cultivated in an artificial greenhouse at 20-30°C, 16 hours of light, 8 hours of darkne...
Embodiment 2
[0025] Embodiment 2, compound improves the biological activity assay of maize drought tolerance
[0026] Select 400 seeds of corn (Zheng Dan 958, purchased from Beijing Si Nong Seed Industry Co., Ltd.) with neat size and plumpness, and use 300mL 10% H 2 o 2 Soak in aqueous solution for 15 minutes, rinse the seeds with a large amount of sterile water after disinfection, in order to remove residual H 2 o 2 , soaked in 300mL sterile water for 24h to accelerate germination. Sow the germinated corn seeds into quartz sand, cover them with black plastic bags, and replenish water regularly. Remove the black plastic bag after the seeds germinate, and carry out normal cultivation. The cultivation conditions are 16 hours of light, 8 hours of darkness, and a temperature of 23-28°C. When the seedlings grow to one leaf and one heart, they are transplanted for hydroponics. Replace every three days. When the seedlings grow to two leaves and one center, spray water, 100μMABA and the compo...
Embodiment 3
[0033] Embodiment 3, compound improves the biological activity assay of cotton (CCSI41, purchased from China Cotton Research Institute) salt tolerance
[0034] All the experiments were completed in the light culture room of the transgenic base in the West Campus of China Agricultural University, using hydroponic experiments in the greenhouse, arranged in random blocks, a total of 5 treatments, and repeated 5 times. The culture conditions in the culture room were light for 14h (30°C), dark for 10h (25°C), light intensity 450μmol m -2 the s -1 . Cotton seeds were delinted with concentrated sulfuric acid and treated with 10% H 2 o 2 Disinfect by soaking in aqueous solution for 15 minutes. The sterilized seeds were soaked in sterilized water for 12 hours under greenhouse conditions, and germinated in the pre-high-temperature sterilized sand. After the two cotyledons are fully expanded (6d), select seedlings with consistent growth and transplant them into plastic pots of 35cm×...
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