Application of Bacillus subtilis in degrading protein in tobacco products
A technology of bacillus subtilis and tobacco products, which is applied in the application field of bacillus subtilis xp strains in degrading proteins in tobacco products, can solve problems such as differences in technical effects, achieve improved smoking evaluation effects, good application value and application prospects, The effect of accelerating the aging process
Active Publication Date: 2018-08-03
CHINA TOBACCO HENAN IND
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Problems solved by technology
However, because the existing research is still relatively limited, and there are certain differences in technical effects, it is still of great significance to develop new microbial agents that can reduce the protein content in tobacco products.
Method used
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[0024] In order to use the Bacillus subtilis xp strain to degrade proteins in tobacco products, it is first necessary to appropriately amplify and cultivate the strain, and prepare it to contain a certain amount of active bacterial agent before it can be used. The specific preparation method of the bacterial agent is as follows.
[0025] (1) Strain activation, using an inoculation loop to pick up the strains on the slant medium (the formula is the same as that of LB liquid medium, only add 15g / L agar powder), place them in LB liquid medium for suspension culture, and incubate at 30°C for 12 hours Activation of bacteria;
[0026] (2) Strain expansion, transfer the bacterial liquid after the activation of the strain in step (1) to LB liquid culture medium, and cultivate it on a shaker at 35°C until OD 600 =3.5 or so, it can be used as bacterial agent for degrading tobacco products after being evenly suspended.
[0027] When the above bacterial agent is specifically used to degr...
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The invention belongs to the technical field of tobacco preparation, in particular to an application of bacillus subtilis xp strain in degrading protein in tobacco products. The Bacillus subtilis is a Bacillus subtilis xp strain, which is now preserved in the China Center for Type Culture Collection, with a preservation number of CCTCC No: M 2011452; the strain is used to reduce the protein content in tobacco products; when applied, the Bacillus subtilis The Bacillus xp strain was prepared into an inoculum, which was evenly sprayed on the surface of tobacco products at a mass ratio of 0.7% of tobacco products, and then stored and aged for 30-40 days at 30°C and 55-65% humidity. The invention proves that it can effectively reduce the protein content in tobacco products through the selection of specific Bacillus subtilis strains, improve the grade of tobacco, and accelerate the aging process of tobacco leaves while ensuring the aging effect.
Description
technical field [0001] The invention belongs to the technical field of tobacco preparation, in particular to an application of bacillus subtilis xp strain in degrading protein in tobacco products. Background technique [0002] Bacillus subtilis is a kind of Bacillus genus, which is widely distributed in soil and decayed organic matter, and is easy to reproduce in subtilis juice. The surface of its colony is rough and opaque, stained white or yellowish, and can use protein, various sugars and starch to decompose tryptophan to form indole, which is widely used in genetic research. In addition, Bacillus subtilis itself can synthesize enzymes such as α-amylase, neutral protease, lipase, cellulase, etc., and has good application prospects in degrading complex macromolecular compounds. Therefore, Bacillus subtilis has gradually become a A hotspot of attention and research. [0003] Tobacco leaf fermentation is also called tobacco leaf alcoholization. Its essence is the process o...
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IPC IPC(8): C12N1/20
CPCA24B15/20
Inventor 杨宗灿马宇平张展陈芝飞于建春彭玉富周浩郝辉聂聪
Owner CHINA TOBACCO HENAN IND