An organic polymeric monolithic column that specifically recognizes ochratoxin and its preparation method

A technology for ochratoxin and specific recognition, which is applied in separation methods, chemical instruments and methods, and other chemical processes, and can solve problems such as large material consumption, polar solvent interference, and rigid recognition "holes are easily destroyed or deformed" , to achieve more sites, improve stability, and improve the efficiency of modification and bonding
CN106731005BActive Publication Date: 2018-12-25FUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FUZHOU UNIV
Publication Date
2018-12-25

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Abstract

The invention discloses an organic polymerized monolithic column capable of specially recognizing ochratoxin, and a preparation method of the organic polymerized monolithic column. According to the monolithic column, a polymerized monolithic column fixed phase having a branched structure of an epoxy group is firstly formed by polymerizing an epoxy cyclosiloxane acrylate monomer and an acrylate cross-linking agent, then post-column ring-opening addition is carried out, and an aptamer capable of specially recognizing the ochratoxin is prepared by covalent bonding. The aptamer and an epoxy group, distributed on the surface of the epoxy cyclosiloxane polymerized monolithic column matrix fixed phase in a branching way, are subjected to further ring-opening addition, so that condensation coupling is realized; the prepared polymerized monolithic column matrix fixed phase has a cyclosiloxane octatomic ring-shaped structure and the epoxy group distributed in a branching way, thus being stable in structure; the organic polymerized monolithic column is more in sites acting with 5'-amino aptamer and small in steric hindrance, and can realize efficient bonding with ochratoxin A aptamer, thus being suitable for the specific recognition of the ochratoxin A.
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Description

technical field

[0001] The invention belongs to the field of preparation of monolithic column functional materials, and in particular relates to an organic polymeric monolithic column for specifically recognizing ochratoxin and a preparation method thereof. Background technique

[0002] Capillary monolithic columns are a new type of chromatographic micro-separation columns. Currently, capillary monolithic columns are mainly based on the principle of reversed-phase interaction or hydrophilic interaction for separation. In terms of specific analysis, affinity chromatographic columns use ligands with specific recognition functions immobilized on chromatographic stationary phases to make specific interactions with analytes and achieve separation from other substances in the sample.

[0003] Molecularly imprinted polymers (MIPs) have become the main research object of selective monolithic column materials due to their recognition principle similar to the "key-lock" interaction. ...

Claims

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