Preparation method of salvianolic acid A salt
A salvianolic acid and a technology for extracting salvianolic acid are applied in the field of preparation of salvianolic acid A salt, which can solve problems such as complex process flow, unsuitability for industrial production or preparation, and high production cost, and achieve high purity, which is conducive to development and production. Application, the effect of not being easily oxidized
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Embodiment 1
[0033] Mix 200 kg of raw material Salvia miltiorrhiza with 2000 L of ethanol aqueous solution with a volume fraction of 50%, extract at 82° C. for 2 h, collect the filtrate by filtration, re-extract the filter residue 3 times, combine the obtained filtrates each time, and obtain the salvia miltiorrhiza extract; concentrate the obtained salvia miltiorrhiza extract to 600L, adjust the pH to 4.5 with hydrochloric acid solution, heat up to 120°C for 4 hours, then cool to room temperature, filter, and the filtrate is separated by HPD-100 macroporous resin column chromatography, firstly eluted with water and 20% ethanol aqueous solution to remove impurities , and then eluted with 50% ethanol aqueous solution by volume fraction, collected the eluate containing salvianolic acid A, concentrated until it had no alcohol smell, and continued to separate polyamide column chromatography, and first eluted with water and 35% ethanol aqueous solution by volume fraction to remove Impurities, the...
Embodiment 2
[0035] Mix 200 kg of raw material Salvia miltiorrhiza with 2000 L of ethanol aqueous solution with a volume fraction of 50%, extract at 82°C for 2 hours, collect the filtrate by filtration, re-extract the filter residue twice, and combine the filtrates obtained each time to obtain the salvia miltiorrhiza extract; concentrate the obtained salvia miltiorrhiza extract to 400L, adjust the pH to 4.4 with hydrochloric acid solution, heat up to 120°C for 2.5h, then cool to room temperature, filter, the filtrate is separated by HPD-300 macroporous resin column chromatography, and firstly eluted with water and 20% ethanol aqueous solution Then use 50% volume fraction ethanol water solution to elute, collect the eluate containing salvianolic acid A, concentrate until it has no alcohol smell, then proceed to polyamide column separation, and first use water, volume fraction 35% ethanol water solution to elute Remove impurities, then elute with 75% ethanol aqueous solution, collect the elua...
Embodiment 3
[0037] Mix 200 kg of raw material Salvia miltiorrhiza with 2000 L of ethanol aqueous solution with a volume fraction of 50%, extract at 82°C for 2 hours, collect the filtrate by filtration, re-extract the filter residue twice, and combine the filtrates obtained each time to obtain the salvia miltiorrhiza extract; concentrate the obtained salvia miltiorrhiza extract to 400L, adjust the pH to 4.6 with hydrochloric acid solution, heat up to 120°C for 3 hours, then cool to room temperature, filter, and the filtrate is separated by 1400 macroporous resin column chromatography, firstly eluted with water and 20% ethanol aqueous solution to remove impurities, and then Elute with 50% ethanol water solution by volume fraction, collect the eluate containing salvianolic acid A, concentrate until it has no alcohol smell, and then proceed to polyamide chromatography column separation, first elute with water and 35% ethanol water solution to remove impurities, Then elute with 75% ethanol aque...
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