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Method for breeding chlorella phytoplankton from cyanobacterial bloom

A technology of cyanobacteria blooms and chlorella, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, complicated process, and many operation steps, and save labor costs and raw materials. Inexpensive, simple cultivation process

Active Publication Date: 2017-05-31
NANJING INST OF GEOGRAPHY & LIMNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these media need to be specially configured, and the cost is relatively high. At the same time, because the cultivation step needs to be inoculated with Chlorella algae, there are many operating steps and the process is relatively complicated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In warm June, cyanobacterial blooms (predominant species of cyanobacteria Microcystis) were collected from Taihu Lake where cyanobacteria blooms occurred, and the concentrated cyanobacterial blooms were obtained by filtering with a 50-mesh filter screen. It is placed in a 10L wide-mouth glass bottle in a glass greenhouse, and the volume of the cyanobacterial pulp in the bottle reaches 8L. Then, the cyanobacterial slurry in the bottle is aerated, and the aeration uses the method of connecting the air stone on the air pump. A total of 1 cylindrical air stone is placed in the bottle (the diameter of the air stone is 3cm, and the height of the air stone is 6cm). The air stone is placed at the bottom of the bottle, and the air emerges from the bottom of the bottle through the air stone to the surface of the bloom to form bubbles, which are easy to burst in an instant. The rate of bubble formation is 10 to 20 per second. Thus, a vertical aerobic area is formed in the area wh...

Embodiment 2

[0028] In hot July, the cyanobacterial blooms (the dominant species is the cyanobacteria Microcystis) were collected from the culture pond where cyanobacteria blooms occurred, and the concentrated cyanobacterial blooms were obtained by filtering with a 200-mesh filter screen. At this time, the cyanobacterial blooms were Slurry, abbreviated as cyanobacteria pulp, is placed in a 70L glass cylinder in a glass greenhouse, and the volume of cyanobacterial pulp in the cylinder reaches 50L. Then aerate the cyanobacterial slurry in the tank. The aeration uses the method of connecting the air stone on the air pump. A total of 1 cylindrical air stone (air stone diameter 3cm, height 6cm) is placed in the tank. The air stone is placed at the bottom of the cylinder, and the air emerges from the bottom of the cylinder through the air stone to the surface of the blooming slurry to form bubbles, which are easy to burst in an instant. The rate of bubble formation is 15 to 25 per second. There...

Embodiment 3

[0032] In warm August, cyanobacterial blooms (predominant species of cyanobacteria Microcystis) were collected from Taihu Lake where cyanobacteria blooms occurred, and the concentrated cyanobacterial blooms were obtained by filtering with a 100-mesh filter screen. It is placed in an outdoor 100L plastic white bucket, and the volume of cyanobacterial pulp in the bucket is 30L. Then, aerate the cyanobacterial slurry in the barrel. The aeration uses the method of connecting air stones on the air pump. A total of 2 cylindrical air stones are placed in the tank (the diameter of the air stones is 3cm, and the height of the air stones is 6cm). The air stone is placed at the bottom of the barrel, and the air emerges from the bottom of the barrel through the air stone to the surface of the blooming slurry to form bubbles, which are easy to burst in an instant. The rate of bubble formation is 15 to 25 per second. Thus, two vertical aerobic areas are formed in the area where the air pas...

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Abstract

The invention discloses a method for breeding chlorella phytoplankton from cyanobacterial bloom. The method comprises the following steps: acquiring the cyanobacterial bloom from a water body in which the cyanobacterial bloom occurs and properly concentrating the cyanobacterial bloom; aerating, rotting and degrading the concentrated cyanobacterial bloom at 18-45 DEG C; then adding running water to dilute a total phosphor concentration to 5.0-15.9mg / L after the cyanobacterial bloom is degraded for 25-40 days until the water body becomes odorous and is represented in black brown or dark brown, and adding a mineral substance mixture which is composed of calcium salt, magnesium salt, iron salt and potassium salt; and conducting cultivation for several days, so that chlorella, which is high in concentration and purity, is obtained. With the application of the method, the cyanobacterial bloom can be transformed into the chlorella phytoplankton, so that innocent treatment and utilization of the cyanobacterial bloom are promoted and transformation of nutrient substances in the water body is accelerated; the method can be directly used for processing the cyanobacterial bloom; and in addition, the culture method provided by the invention is simple and convenient to implement; therefore, a good way is provided for resource utilization of the cyanobacterial bloom.

Description

technical field [0001] The invention relates to a method for culturing phytoplankton, in particular to a method for cultivating phytoplankton of the Chlorophyta phylum Chlorella by utilizing cyanobacterial blooms. Background technique [0002] The eutrophication of water bodies leads to the frequent occurrence of algal blooms all over the world, and the most common algal blooms in China are cyanobacterial blooms, especially those of the cyanobacteria phylum Microcystis. This bloom has harmful effects on drinking water sources, shipping, aquaculture, landscapes, tourism, and more. The current treatment method for cyanobacterial blooms is mainly as fertilizer. Freshwater eutrophic lakes in China, such as Taihu Lake, Dianchi Lake and other cyanobacteria blooms, have huge biomass. Although cyanobacterial blooms contain algal toxins, they are rich in nutrients. In addition to being rich in protein, they also contain fat, carbohydrates, minerals, etc. substances and other nutrie...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 秦伯强高光张运林汤祥明许海董百丽
Owner NANJING INST OF GEOGRAPHY & LIMNOLOGY
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