A kind of temperature-sensitive bacterial strain and application for producing polyunsaturated fatty acid

An unsaturated fatty acid, temperature-sensitive technology, applied in the field of bioengineering, can solve the problems of abnormal physiological metabolism of microorganisms, weakening, different temperature of protein functions and regulation methods, etc.

An unsaturated fatty acid, temperature-sensitive technology, applied in the field of bioengineering, can solve the problems of abnormal physiological metabolism of microorganisms, weakening, different temperature of protein functions and regulation methods, etc.

CN106754403BActive Publication Date: 2020-06-26TIANJIN UNIV OF SCI & TECH
0 Cites 0 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of temperature-sensitive bacterial strain and application for producing polyunsaturated fatty acid
  • A kind of temperature-sensitive bacterial strain and application for producing polyunsaturated fatty acid
  • A kind of temperature-sensitive bacterial strain and application for producing polyunsaturated fatty acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Screening and Identification of a Temperature Sensitive Strain

[0036] 1. Plate Screening

[0037] Put 1g of the soil sample collected from the snow mountain in Tibet into a test tube filled with 9mL of sterile saline, shake it well and then dilute it to 10 -5 . Because the snow mountain is in the alpine area under natural conditions, the temperature is low. Take 0.2 mL of the above-mentioned dilutions of each gradient to spread on a PDA plate, and invert and culture at a constant temperature of 20°C until a single colony grows.

[0038] 2. Identification of 18S rDNA

[0039] According to the strains of single colony screened out, the specific primers of fungal 18S rDNA were synthesized, the DNA was amplified, and the PCR products were purified and sequenced.

[0040] Primer sequences used in Table 1

[0041]

[0042] Proceed as follows:

[0043] 1. PCR amplification: the fungal ITS1 / ITS4 universal primers were selected for PCR amplification (Table 1).

[004...

Embodiment 2

[0055] Analysis of unsaturated fatty acids in fermentation products of Mortierella alpina

[0056] Inoculate the Mortierella alpina strain on a PDA plate, culture in the dark at 25°C for 4-5 days, wash the spores and adjust the spore concentration to 1-2×10 5 / ml was inoculated into the seed medium, cultivated at 25°C and 200rpm for 24h, and inoculated into the fermentation medium at 1% inoculum size and fermented at 25°C and 200rpm for 5d. After the fermentation, the bacteria were collected by vacuum filtration, and the bacteria were dried at 50°C to constant weight.

[0057] The medium formula is as follows:

[0058] Seed medium, calculated in g / L: glucose 30g, yeast extract 6g, KH 2 PO 4 2g, MgSO 4 ·7H 2 0 0.5g, NaNO 3 2g, the rest is distilled water, pH=6.0, sterilized at 115°C for 20min.

[0059] Fermentation medium, calculated in g / L: glucose 50g, yeast extract 10g, KH 2 PO 4 2g, MgSO 4 ·7H 2 01g, the rest is distilled water, pH=6.0, sterilized at 115°C for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a temperature-sensitive bacterial strain for producing polyunsaturated fatty acids, the preservation number is CGMCC 11808, and through 18S rDNA identification, the bacterial strain is a temperature-sensitive filamentous fungus of the genus Mortierella. The growth temperature is 25°C, and the temperature sensitive range is 25°C-28°C. After 18S rDNA identification, the strain is Mortierella alpina. The strain can grow well at 20°C-25°C. When the temperature is higher than At 26°C, the hyphae cannot extend normally. When the temperature is higher than 27°C, the bacterial colony becomes significantly smaller; when the temperature is higher than 30°C, the bacteria do not grow at all. Under the condition of 25°C, the strain was fermented with fermentation medium, and it was found that it could produce various unsaturated fatty acids.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a temperature-sensitive bacterial strain for producing polyunsaturated fatty acids and its application. Background technique [0002] Appropriate temperature is a necessary condition for the growth of microorganisms, and different temperatures may have certain effects on the energy metabolism pathways of microorganisms. Different microorganisms have different optimum growth temperatures, so their genetic material will also have certain differences. The temperature of the external environment of microorganisms also affects the ratio of saturated fatty acids and unsaturated fatty acids in the phospholipids of microbial cell membranes. When the ambient temperature drops, the proportion of unsaturated fatty acids will increase, while the proportion of saturated fatty acids will decrease. Because unsaturated fatty acids contain more double bonds than saturated fatty acids and have a lo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
26 Jun 2020
Publication
CN106754403B
IPC
C12N1/14; C12P7/64; C12R1/645
CPC
C12P7/6427; C12N1/145; C12R2001/645
Inventors
刘浩; 王海霞