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Urine nucleic acid fast extraction kit

A urine nucleic acid and kit technology, applied in the biological field, can solve the problems of toxicity of chemical substances, time-consuming non-negligible, error-prone and other problems, achieve a high degree of automation, reduce the time required for extraction, reduce human errors and experimental errors Effect

Inactive Publication Date: 2017-05-31
GUANGZHOU HEAS BIOTECH CO LTD
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Problems solved by technology

[0003] Although the traditional method of extracting nucleic acid with phenol chloroform can obtain better quality nucleic acid, the chemical substances used in it have non-negligible toxicity, and the whole extraction process takes a long time, wasting a lot of time, manpower and material resources
Furthermore, the now popular column extraction nucleic acid extraction reagents can also obtain good quality nucleic acids with negligible toxicity, but the time-consuming can also not be ignored
[0004] In addition to toxicity and time factors, the addition and discarding of various washing solutions are inevitable in the operation of extracting nucleic acids with these two reagents. This process is extremely easy to cause cross-contamination between samples
When the sample size is large, it is easy to make mistakes, resulting in low work efficiency

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Embodiment Construction

[0028] 1. Preparation method of urine nucleic acid rapid extraction kit:

[0029] a. The configuration of the lysis binding solution: first add the weighed guanidine isothiocyanate into the volumetric flask and add an appropriate amount of sterilized pure water to dissolve, adding Tris-HCl with a final concentration of 10mmol / L and a pH of 3.3; The concentration is 5mmol / L sodium hydroxide; after the guanidine isothiocyanate is completely dissolved, add 13% NP-40 and mix well, then add sterile water to the required volume.

[0030] b. Configuration of washing solution 1: first add a small amount of sterile water into the volumetric flask, add Tris-HCl with a concentration of 60mmol / L and a pH of 7.5; add ethanol with a final concentration of 40%; mix well, and then add sterile water to required volume.

[0031] c. Configuration of washing solution 2: first add a small amount of sterile water to the volumetric flask, and add a final concentration of 5mmol / LCaCl 2 ; Add final ...

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Abstract

The invention discloses a urine nucleic acid fast extraction kit. The urine nucleic acid fast extraction kit comprises a combined lysate, protease K, magnetic beads, a detergent 1, a detergent 2 and an eluent. The kit adopts a 96-hole pre-packaged deep hole plate mode and can be matched with a nucleic acid automatic extractor smart32 made by Da An Gene Co., Ltd. Of Sun Yat-Sen University, so that the kit automatically extracts nucleic acid, and can massively, quickly and effectively extract DNA in the urine.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a urine nucleic acid rapid extraction kit. The invention also relates to a method for extracting DNA in urine by using the magnetic bead method. Background technique [0002] In the diagnosis of modern diseases, nucleic acid plays a very important role, and the role of DNA is more prominent. Extracting DNA from urine for disease detection is playing an increasingly important role in modern disease detection. In the process of PCR molecular diagnosis, the total copy number and quality of DNA obtained will directly affect the results of subsequent testing experiments. Urine samples contain high levels of PCR inhibitors, including urea, uric acid and other impurities. These impurities have a great inhibitory effect on conventional nucleic acid extraction, making the efficiency of nucleic acid extraction extremely low. Therefore, obtaining effective and reliable DNA from urine is the k...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1013C12Q2563/143C12Q2563/149C12Q2523/308
Inventor 刘淑园陈华云肖湘文丁渭张天海刘孝礼曾烨黄爽
Owner GUANGZHOU HEAS BIOTECH CO LTD
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