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SNP (single nucleotide polymorphism) marker for Chinese cabbage type crop bolting and flowering identification

A crop and marker technology, applied in the direction of recombinant DNA technology, DNA/RNA fragments, etc., can solve the problem that the BrFLC5 gene cannot be transcribed normally

Inactive Publication Date: 2017-05-31
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0005] Zhang Xueming et al. (2014) found that there is a Pi3+1 (G-A) mutation in exon 3 of BrFLC5, which leads to the abnormal transcription of BrFLC5 gene

Method used

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  • SNP (single nucleotide polymorphism) marker for Chinese cabbage type crop bolting and flowering identification
  • SNP (single nucleotide polymorphism) marker for Chinese cabbage type crop bolting and flowering identification
  • SNP (single nucleotide polymorphism) marker for Chinese cabbage type crop bolting and flowering identification

Examples

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Effect test

Embodiment 1

[0071] Example 1: Identification of Pi3+1(G-A) variation in BrFLC5 in Chinese cabbage varieties using SNP markers developed based on KASP technology.

[0072] (1) Extraction of whole-genome DNA of Chinese cabbage varieties: take the leaf tissues of Chinese cabbage varieties to be tested (281 parts of Chinese cabbage), extract whole-genome DNA by CTAB method, and dilute the whole-genome DNA of each Chinese cabbage variety to 15 ng / μl;

[0073] (2) Dilute primers: Dilute according to the primer dilution information provided by the synthetic primer company. First, centrifuge the dry powder primers to prevent splashing of the dry powder primers near the nozzle when opening the cap. According to the primer dilution information, dilute the forward FAM fluorescent primers, The concentration of forward VIC fluorescent primer and reverse primer (sequence information as shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3 in the sequence table) was diluted to 10 μM;

[0074] (3) Prepare...

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Abstract

The invention relates to an SNP (single nucleotide polymorphism) marker for Chinese cabbage type crop bolting and flowering identification. The SNP marker is nucleotide A or G positioned at a Pi3+1 bit of an exon of a BrFLC5 gene on A03 chromosome of a Chinese cabbage type crop. The bolting and flowering time of a GG genotype individual of the SNP marker is later than that of GA and AA genotype individuals. The SNP marker can identify functional variation sites of the Chinese cabbage type crop for controlling bolting and flowering related genes BrFLC5 in a high-throughput manner, target site genotype detection accuracy can reach up to a hundred percent according to inspection, the SNP marker has high efficiency and accuracy, is simple and convenient to operate and low in cost, and marker assisted selection can be effectively performed in bolting-resistant molecular breeding of the Chinese cabbage type crop.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding of cabbage crops, and in particular relates to a SNP marker for identification of bolting and flowering of cabbage crops. Background technique [0002] Cabbage crops include various types of vegetable crops such as Chinese cabbage, Chinese cabbage, cabbage stalks, mizuna, wutah, and turnip, as well as cabbage-type rapeseed as an oil crop. They are widely cultivated all over the world and have a long history. Bolting and flowering is one of the important agronomic traits of cabbage crops, and the time of bolting and flowering directly affects the commercial quality of cabbage crops. In the production and cultivation of Chinese cabbage crops in spring and autumn and winter in alpine regions, encountering low temperature often leads to early bolting and flowering, which makes it completely lose its commerciality and becomes a major problem that plagues production. Improving the early bolt...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 武剑郗希王晓武梁建丽程锋
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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