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Photoelectrochemical biosensor and preparation method thereof

A biosensor and photoelectrochemical technology, applied in the field of biosensing, can solve the problems of difficult miniaturization of photoelectrochemical biosensors, and achieve the effects of simple structure, small device size and high sensitivity

Active Publication Date: 2017-05-31
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a photoelectrochemical biosensor and a preparation method thereof, thereby further improving the sensitivity of the photoelectrochemical biosensor and solving the problem that the existing photoelectrochemical biosensor is not easy to miniaturize

Method used

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  • Photoelectrochemical biosensor and preparation method thereof
  • Photoelectrochemical biosensor and preparation method thereof
  • Photoelectrochemical biosensor and preparation method thereof

Examples

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Effect test

Embodiment 1

[0047] Example 1 Photoelectrochemical DNA sensor based on organic electrochemical transistor

[0048] Principle: The gate electrode is an ITO electrode assembled with cadmium sulfide quantum dots (CdS QDs). Under light conditions, when the energy of the light is greater than the energy required for the electron transition in CdS, the electrons in the valence band in CdS will transition to the conduction band. , forming electron-hole pairs. When the electrons in the conduction band are injected into the electrode, the electron donor in the solution provides electrons to the holes in the valence band, which will form a photocurrent. The generation of this current will reduce the potential of the electrolyte / gate electrode interface, thereby increasing the applied on the OECT device. effective gate voltage. The channel current of OECT is given by the following equation:

[0049]

[0050] where q stands for electron charge, µ stands for hole mobility, represents the initial...

Embodiment 2

[0062] Example 2 Photoelectrochemical immunosensor based on organic electrochemical transistor

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Abstract

The present invention discloses a photoelectrochemical biosensor comprising an electrolytic cell, electrolyte provided in the electrolytic cell, an organic electrochemical transistor provided in the electrolytic cell, and a gate electrode provided in the electrolytic cell, wherein the organic electrochemical transistor comprises a substrate, a source electrode and a drain electrode disposed on the substrate, and an organic semiconductor thin film layer coating the substrate and connected with the source electrode and the drain electrode; the gate electrode is modified with a photoelectric active semiconductor material as a sensitive functional layer of the sensor. The photoelectrochemical biosensor of the invention has extremely high sensitivity, the structure is simple and the device size is small, and the problem that the photoelectrochemical biosensor is not easy to miniaturize is solved. The photoelectrochemical biosensor of the invention has universality in the field of biological detection, and can be widely applied in the biosensor field such as enzyme biosensor, cell biosensor and the like, besides applied to DNA sensor and immune sensor.

Description

technical field [0001] The invention relates to the technical field of biosensing, in particular to a photoelectrochemical biosensor and a preparation method thereof. Background technique [0002] Photoelectrochemical (PEC) biosensing technology is a biosensing technology developed on the basis of electrochemical analysis methods. Due to its high sensitivity, low price and simple equipment, it has been widely used in enzyme biosensing. , DNA sensing, immune sensing, and cell sensing and other biosensing. The detection principle of PEC is based on the biorecognition between the recognition element and the target molecule under light to produce a change in the corresponding electrical signal. At present, the detection of the signal in the PEC biosensor is mainly through the electrochemical workstation, and a three-electrode test system consisting of a working electrode, a reference electrode, and a counter electrode is established to detect the magnitude of the photocurrent. ...

Claims

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Application Information

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IPC IPC(8): G01N27/414
CPCG01N27/4145
Inventor 林鹏宋家俊魏伟伟柯善明曾燮榕
Owner SHENZHEN UNIV
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