Method for detecting multiple fat-soluble vitamins in feed

A technology for fat-soluble vitamins and vitamins, which is applied in the field of chromatographic detection and chromatographic detection and analysis of vitamins. It can solve the problems of long time consumption, lack and low efficiency of detection and analysis of compound feed, and achieve accurate and reliable analysis results, high detection and analysis accuracy, good separation effect

Active Publication Date: 2017-05-31
NEW HOPE LIUHE +1
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Problems solved by technology

[0013] The purpose of the present invention is to overcome the lack of simultaneous detection and analysis methods for multiple fat-soluble vitamins in compound feeds in complex s

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  • Method for detecting multiple fat-soluble vitamins in feed
  • Method for detecting multiple fat-soluble vitamins in feed
  • Method for detecting multiple fat-soluble vitamins in feed

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Embodiment Construction

[0045] The present invention will be further described in detail below in conjunction with test examples and specific embodiments. However, it should not be understood that the scope of the above subject matter of the present invention is limited to the following embodiments, and all technologies realized based on the content of the present invention belong to the scope of the present invention.

[0046] 1 Instruments and reagents

[0047] (1) Agilent 1260-6460 liquid chromatography-triple quadrupole tandem mass spectrometer with electrospray ion source ESI (Agilent Corporation, USA);

[0048] (2) AB-135 electronic analytical balance (Mettler Toledo Shanghai Co., Ltd.).

[0049] (3) LD5-2B centrifuge (Beijing Jingli Centrifuge Co., Ltd.).

[0050] (4) KQ-500DE CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0051] (5) Vortex mixer MS3 (IKA, Germany).

[0052] (6) Acetonitrile, methanol (chromatographically pure, Merck.

[0053] (7) Formic acid, ammoniu...

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Abstract

The invention discloses a method for detecting multiple fat-soluble vitamins in a feed. The method comprises the steps of (1) preparing multi-vitamin standard solution which comprises at least two of vitamin A acetate, vitamin D3 and vitamin E acetate according to the detection requirements, and detecting the standard solution by adopting an HPLC-MS/MS to obtain a standard curve equation; (2) weighing a sample to a brown volumetric flask, adding an extract solution for ultrasonic extraction for 10-30 minutes, cooling to constant volume, centrifuging, filtering and processing by adopting a 0.22micron of organic phase filter membrane and carrying out analytical test on a chromatographic parameter and a mass spectrum detection parameter same as those in the step (1); and (3) calculating the content of multiple vitamins in the sample according to the detection results in the steps (1) and (2). According to the method for detecting multiple fat-soluble vitamins in the compound feed at the same time, detection and analysis of multiple different vitamins are completed in one detection and analysis process.

Description

technical field [0001] The invention relates to a chromatographic detection and analysis method, in particular to a chromatographic detection method for detecting vitamins, which has good detection and analysis efficiency for fat-soluble vitamins, and belongs to the technical field of chemical component analysis. Background technique [0002] Fat-soluble vitamins include VA, VD, VE, and VK. Because their chemical structures contain mostly hydrophobic hydrocarbon chains, they are insoluble in water and easily soluble in organic solvents. Due to the difference in solubility, its detection method is also different from that of water-soluble vitamins, especially the type of reagent used in sample pretreatment should be based on organic solvents. [0003] At present, the detection methods for water-soluble vitamins are relatively complete, such as titration, colorimetry, spectrophotometry, and the high-performance liquid chromatography and reversed-phase high-performance liquid c...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 朱正鹏梁玉树何开蓉易锡斌张遨然汪春霞王虎周丽黄李蓉梁秋菊
Owner NEW HOPE LIUHE
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