Bladder cancer diagnosis marker and application thereof and diagnosis kit

A diagnostic marker and diagnostic kit technology, applied in the field of biomedicine, can solve the problems of easy missed diagnosis and misdiagnosis of bladder cancer in situ, invasive cost of cystoscopy, low detection sensitivity, etc., and achieve good diagnostic efficiency and accuracy. Diagnosis, the effect of high diagnosis result

Inactive Publication Date: 2017-05-31
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cystoscopy is not only invasive but also expensive, and it is often easy to miss and misdiagnose bladder carcinoma in situ
Urinary exfoliation cytology has low sensitivity, especially for low-grade bladder cancer

Method used

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  • Bladder cancer diagnosis marker and application thereof and diagnosis kit
  • Bladder cancer diagnosis marker and application thereof and diagnosis kit
  • Bladder cancer diagnosis marker and application thereof and diagnosis kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Screening for diagnostic markers:

[0031] (1) Collection of serum samples: 34 serum samples from low-grade and high-grade bladder cancer patients were collected as the low-grade bladder cancer group and high-grade bladder cancer group, and 30 serum samples from healthy controls were used as the healthy control group. Samples were stored frozen at -80°C;

[0032] (2) Serum sample pretreatment: Take 100 μL of serum sample after freezing and thawing, add 300 μL methanol (containing 12.5 μg / ml L-2-chloropropanine, used as internal standard) vortex for 1 min, and then place in ice water bath for ultrasound Extract for 10 minutes, centrifuge at 14000g for 15 minutes at 4°C, absorb the supernatant and inject the sample;

[0033] (3) Sample liquid chromatography-mass spectrometry analysis: Serum samples were analyzed by ultra-high performance liquid chromatography and mass spectrometry. The liquid chromatography was an Agilent 1290 Infinity liquid chromatography system with a...

Embodiment 2

[0044] Validation of the diagnostic efficacy of high-grade bladder cancer from low-grade bladder cancer:

[0045] In order to quantitatively evaluate the diagnostic efficacy of the model for high-grade bladder cancer and low-grade bladder cancer, a receiver operating curve (ROC curve, Figure 7 ). The results of the ROC curve in this study showed that the area under the ROC curve calculated from the quantitative data of the three diagnostic markers in 34 cases of high-grade bladder cancer and 34 cases of low-grade bladder cancer reached 0.961, the sensitivity reached 88.2%, and the specificity Up to 91.2%, these results indicate that the combination of these three diagnostic markers has a high accuracy in distinguishing high-grade bladder cancer from low-grade bladder cancer. The distribution of P values ​​is shown in Figure 8 As shown, when reaching the highest sensitivity (88.2%) and specificity (91.2%), the optimal cut-off value of model P is equal to 0.4669, is cut-off ...

Embodiment 3

[0047] Diagnostic kits and diagnostic kits in the diagnosis and differentiation of high-grade bladder cancer and low-grade bladder cancer:

[0048] The diagnostic kit of the present embodiment includes a diagnostic reagent and a diagnostic model, and the diagnostic reagent includes inosine, N-acetyl-N-2-formyl-5-methoxykynurenine and phosphatidylserine (O-18: 0 / 0:0) standard products of the three markers, the diagnostic model is:

[0049]

[0050] Among them, W 肌苷 Represents the peak area of ​​inosine in serum samples detected by liquid chromatography-mass spectrometry, W 犬尿氨酸 Representative peak area of ​​N-acetyl-N-2-formyl-5-methoxykynurenine in serum samples detected by liquid chromatography-mass spectrometry, W 磷脂酰丝氨酸 Represents the peak area of ​​phosphatidylserine (O-18:0 / 0:0) in serum samples detected by liquid chromatography-mass spectrometry, W 内标 Represents the peak area of ​​the internal standard L-2-chlorophenylalanine in the serum samples detected by liquid...

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Abstract

The invention provides a bladder cancer diagnosis marker which comprises inosine, N-acetyl-N-2-formyl-5-methoxy kynurenine and phosphatidylserine (O-18: 0/0: 0). A diagnosis index for a low-level bladder cancer and a high-level bladder cancer can be built through combination of human serum inosine, N-acetyl-N-2-formyl-5-methoxy kynurenine and phosphatidylserine (O-18: 0/0: 0), and accurate and fast diagnosis is carried out on the bladder cancer. The invention further provides application of the bladder cancer diagnosis marker in preparation of the bladder cancer diagnosis kit. In addition, the invention further provides the bladder cancer diagnosis kit. The bladder cancer diagnosis marker is capable of accurately judging the bladder cancer and accurately distinguishing the low-level bladder cancer from the high-level bladder cancer; the accuracy of the diagnosis result is high; and the quantizable, objective and sensitive clinical diagnosis index is provided for diagnosis of the bladder cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a bladder cancer diagnostic marker, its application and a diagnostic kit. Background technique [0002] Bladder cancer is the most common malignant tumor in the urinary system in my country. Bladder cancer can be divided into low-grade and high-grade bladder cancer according to how different the tumor cells are from normal cell structures. A tumor is considered high-grade or HG if it is characterized by its high invasiveness and high degree of malignancy to invade other tissues, or low-grade or LG when it is characterized by its low degree of malignancy or invasive potential tumor. Low-grade bladder cancer can usually be cured by endoscopic local excision, while high-grade bladder cancer usually requires radical cystectomy because of its high degree of malignancy, easy recurrence, and distant metastasis. Currently, cystoscopy and urine exfoliation cytology are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N33/574G06F19/00
CPCG01N30/88G01N33/57484G01N2030/8822G16H50/50
Inventor 谭光国孟平周倩吴红袁建林秦卫军
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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