Antibody preservation solution and preparation method thereof

A technology for preserving solution and antibodies, applied in the biological field, can solve the problems of antibody denaturation, reduce antibody activity, affect antibody activity and use effect, etc., and achieve high activity and prevent proteolysis.

Active Publication Date: 2017-06-06
广州桂雨生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of antibody utilization, whether it is properly preserved or not directly affects the activity and use effect of the antibody. For most antibodies, it is usually stored at low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The preparation method of the antibody preservation solution is as follows: add 5% trehalose, 0.007% sodium azide, 40% glycerol, 0.0022% of the first polypeptide, 0.0022% of the second polysaccharide to the pH 7.2, 50mmol / L Tris-HCl buffer solution Peptides were mixed evenly to prepare antibody preservation solution. Among them, the concentrations of trehalose, sodium azide, glycerin, the first polypeptide, and the second polypeptide are mass ratios, specifically g / L; the amino acid sequence of the first polypeptide is Val-Pro-Thr-Gly-Ala -Pro, the amino acid sequence of the second polypeptide is Ile-Pro-Gly-Pro-Val-Gly.

[0016] The activities of the antibodies preserved in different ways are tested below to illustrate the effectiveness of the antibody preservation solution in this embodiment. Antibody preservation methods include: using the antibody preservation solution prepared in Example 1 to preserve antibodies; existing room temperature antibody preservation sol...

Embodiment 2

[0021] The preparation method of the antibody preservation solution is as follows: add 5.3% trehalose, 0.008% sodium azide, 43% glycerol, 0.0023% the first polypeptide, 0.0023% the second multi Peptides were mixed evenly to prepare antibody preservation solution. Among them, the concentrations of trehalose, sodium azide, glycerin, the first polypeptide, and the second polypeptide are mass ratios, specifically g / L; the amino acid sequence of the first polypeptide is Val-Pro-Thr-Gly-Ala -Pro, the amino acid sequence of the second polypeptide is Ile-Pro-Gly-Pro-Val-Gly.

[0022] The activities of the antibodies preserved in different ways are tested below to illustrate the effectiveness of the antibody preservation solution in this embodiment. Antibody preservation methods include: using the antibody preservation solution prepared in Example 2 to preserve antibodies; existing room temperature antibody preservation solutions to preserve antibodies; existing low temperature (-20°C...

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Abstract

The invention discloses an antibody preservation solution. The antibody preservation solution is prepared from a buffer solution, 4-6% of trehalose, 0.005-0.01% of sodium azide, 30-50% of glycerol, 0.002-0.0025% of first polypeptide and 0.002-0.0025% of second polypeptide, wherein the buffer solution is 40-100mmol/L Tris-HCl buffer solution with a pH value of 7.0-7.5; the concentrations of the trehalose, the sodium azide, the glycerol, the first polypeptide and the second polypeptide are respectively the mass ratio; the amino acid sequence of the first polypeptide is Val-Pro-Thr-Gly-Ala-Pro; the amino acid sequence of the second polypeptide is Ile-Pro-Gly-Pro-Val-Gly. The invention also discloses a preparation method of the antibody preservation solution. The antibody preservation solution provided by the invention can be stored at room temperature for a long time, and can enable an antibody to maintain higher activity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an antibody preservation solution and a preparation method thereof. Background technique [0002] Antibody refers to the immunoglobulin produced by plasma cells differentiated from B lymphocytes by the body's immune system under the stimulation of antigens, and can specifically bind to corresponding antigens. Because antibodies can specifically and stably bind specific antigens, they are widely used. In the process of antibody utilization, whether it is properly preserved or not directly affects the activity and use effect of the antibody. For most antibodies, it is usually stored at low temperature. Using this method of storage, repeated freezing and thawing of the antibody will lead to denaturation of the antibody, multiple Aggregate production, thereby reducing antibody activity. [0003] How to provide an antibody preservation solution that can be stored for a long time at room...

Claims

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Application Information

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IPC IPC(8): C07K16/00C07K1/00
CPCC07K16/00
Inventor 于志军张希涛韩艳萍
Owner 广州桂雨生物科技有限公司
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