High-precision instant orthogonal optical projection tomography system

A technology of optical projection tomography and imaging system, which is applied in the field of instantaneous orthogonal optical projection tomography system, and can solve the problems of no related reports

Inactive Publication Date: 2017-06-09
SHENZHEN UNIV
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Problems solved by technology

As a result, there have been several studies using fluorescence microscopy to image cell movement in subregions of the zebrafish body in 2D and 3D, but time-lapse cell tracking of the whole zebrafish has not been reported so far.

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  • High-precision instant orthogonal optical projection tomography system

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Embodiment 1

[0020] Embodiment 1: The three-dimensional tracking system of rotating instantaneous orthogonal imaging performs three-dimensional tracking on the immune cells (such as neutrophils) of living model animals, and strives to improve the time-domain accuracy. Compared with the traditional three-dimensional tracking for three-dimensional imaging of samples at each time point, the present invention uses the instantaneous orthogonal imaging system to realize the minimum light radiation (minimum photobleaching and phototoxicity) for three-dimensional tracking of samples, which has little damage to living organisms and provides Biological, medical and pharmaceutical research provides new technical means and has important application value. 3D tracking of neutrophils in transgenic zebrafish embryos based on time-lapse in orthogonal projection angles. Imaging using the interaction of light and biological tissue has always been favored, because optical imaging has low tissue interference,...

Embodiment 2

[0026] Embodiment 2: A transient orthogonal imaging technique is proposed for three-dimensional tracking of sparsely distributed cells in the body, and optical projection tomography is proposed to obtain higher time-delay resolution. Different from the traditional three-dimensional tracking technology that needs to collect three-dimensional space at each time point, the present invention intends to reduce the light radiation to the sample. And use the angle multiplexing OPT system to collect two orthogonal images at different projection angles at the same time, and plan to use the time up to the camera frame rate to locate and track the characteristic target.

[0027] Place the sample on the rotating stage, focus the two orthogonal imaging arms to the rotation axis, select the appropriate system component parameters, laser, CCD, electric control shutter, filter, diaphragm, lens, etc., to build a dual-axis instantaneous orthogonal OPT imaging system. For sample preparation, a ...

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Abstract

The invention discloses a high-precision instant orthogonal optical projection tomography system. Three-dimensional tracking for neutrophile granulocyte in zebra fish embryo can be realized. The collecting rate can reach camera frame rate and the optical radiation to a biological sample is small. Various image processing and recognition algorithms are used for detecting and recognizing the neutrophile granulocyte in the zebra fish embryo, so as to acquire the position change and morphological features of the cells. A basis is established for data analysis of the next step; the mutual relation of the neutrophile granulocyte structure, morphological feature, motion trail, inflammation and drug effect can be realized; the experimental and theoretical basis is established for researching the disease change and the drug effect mechanism.

Description

technical field [0001] The invention relates to the field of optical imaging and image processing pattern recognition, and relates to an instantaneous orthogonal optical projection tomography system. Background technique [0002] Revealing the essence of life and paying attention to human health are the main lines of the development of contemporary science and technology. By imaging biological samples to study the structural characteristics and physiological functions of samples, the purpose of providing objective basis for medical clinical diagnosis can be achieved. Optical projection tomography (Optical Projection Tomography, OPT) technology combines CT technology and microscopy technology, and is a new technology that can perform three-dimensional imaging of fluorescent and non-fluorescent substances. OPT has a higher resolution than nuclear magnetic resonance, which can reach the micron level; it has a larger imaging depth than confocal microscopy imaging, and can image...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00
CPCA61B5/0073A61B5/4845A61B2503/40A61B2503/42
Inventor 胡学娟陈玲玲阮双琛
Owner SHENZHEN UNIV
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