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Artificial cultivation of cordyceps militaris

A technology of Xuefeng Cordyceps and artificial cultivation, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems of limited food intake of larvae, damage to the ecological environment, felling of large greens, etc., and achieve easy large-scale cultivation, technical Easy, low-cost effect

Inactive Publication Date: 2017-06-13
湖南省中医药研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this breeding method, the output of Cordyceps xuefeng needs Daqing, boiled bat moth larvae, and Cordyceps xuefeng strains, which are indispensable. The disadvantage is that a large number of Daqing will be cut down, which will destroy the ecological environment; the compost and bacterial powder are easy to mix Cross-infection, breeding of miscellaneous bacteria; larvae have limited food intake, low infection rate, and long infection time

Method used

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  • Artificial cultivation of cordyceps militaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] S1: selection of larvae: choose the larvae of the boiled bat moth with a size of 4 cm as the host;

[0035] S2: Strain selection: Cordyceps Xuefeng, a fungus of the family Nematodeceps;

[0036] S3: Preparation of Xuefeng Cordyceps strains: Divide the rejuvenated Cordyceps Xuefeng slant strains into soybean-sized pieces, inoculate each 2 pieces into PDA liquid medium, and cultivate at 25°C and 130r / min for 15 days to obtain a fermentation broth. Filtered liquid strains;

[0037] S4: Preparation of nutrient soil: Mix sandy loam soil and peat soil at a ratio of 2:1, then add water to prepare soil with a water content of 15%, and sterilize for 30 minutes at a temperature of 121°C and a pressure of 0.1Mpa;

[0038] S5: Disinfection and inoculation of larvae body surface: Wipe the larvae in S1 with 75% alcohol and put them on the ultra-clean workbench for ultraviolet disinfection for 20 minutes, then use sterile cotton balls dipped in bacteria solution to smear the steriliz...

Embodiment 2

[0043] S1: selection of larvae: choose the larvae of the boiled bat moth with a size of 8 cm as the host;

[0044] S2: Strain selection: Cordyceps Xuefeng, a fungus of the family Nematodeceps;

[0045] S3: Preparation of Xuefeng Cordyceps strains: Divide the rejuvenated Cordyceps Xuefeng slant strains into soybean-sized pieces, inoculate each 3 pieces into PDA liquid medium, and cultivate at 25°C and 130r / min for 15 days to obtain a fermentation broth. Filtered liquid strains;

[0046] S4: Preparation of nutrient soil: Mix sandy loam soil and peat soil at a ratio of 2:1, then add water to prepare soil with a water content of 15%, and sterilize for 30 minutes at a temperature of 121°C and a pressure of 0.1Mpa;

[0047] S5: Larva surface disinfection and inoculation: wipe the larvae in S1 with 75% alcohol and put them on the ultra-clean workbench for UV disinfection for 30 minutes, soak the sterilized larvae in the bacterial solution for 60 seconds;

[0048]S6: Pre-cultivation...

Embodiment 3

[0052] S1: selection of larvae: choose the larvae of the boiled bat moth with a size of 4 cm as the host;

[0053] S2: Strain selection: Cordyceps Xuefeng, a fungus of the family Nematodeceps;

[0054] S3: Preparation of Xuefeng Cordyceps strains: Divide the rejuvenated Cordyceps Xuefeng slant strains into soybean-sized pieces, inoculate each 2 pieces into PDA liquid medium, and cultivate at 25°C and 130r / min for 20 days to obtain a fermentation broth. Filtered liquid strains;

[0055] S4: Preparation of nutrient soil: Mix sandy loam soil and peat soil at a ratio of 2:1, then add water to prepare soil with a water content of 15%, and sterilize for 30 minutes at a temperature of 121°C and a pressure of 0.1Mpa;

[0056] S5: Disinfection and inoculation of the larva body surface: wipe the larvae in S1 with 75% alcohol, put them on the ultra-clean workbench for ultraviolet disinfection for 20 minutes, and then use a disposable syringe to draw 0.2ml of liquid bacteria and inject t...

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Abstract

The invention discloses an artificial cultivation method of forming cordyceps militaris. The nematodes fungi cordyceps taishanensis is parasitized through the manual work on the insect larva of a furuncle moth family. The cultivation method has the positive effects of being simple in operation, low in cost, and easy to promote technically; the insect infection rate and the grass germination rate are high, the nutritional active components of cordyceps militaris are close to the wild cordyceps militaris, the obtained stroma of cordyceps militaris is tight in outer structure, close to the wild cordyceps militaris in appearance, full in stroma, uniform in size and shape, and easy for large-scale cultivations.

Description

technical field [0001] The invention relates to a method for artificially cultivating Cordyceps Xuefeng, in particular to a method for artificially cultivating Cordyceps Xuefeng by infecting live furuncle bat moth larvae with strains of Cordyceps xuefeng and the obtained Cordyceps xuefeng. Background technique [0002] With the continuous improvement of people's living standards and quality of life, and the continuous expansion of the field of knowledge, the demand for functional health care products and food is also increasing. Cordyceps is a class of medicinal and edible fungi with remarkable health effects, the most famous of which is Cordyceps sinensis, and its related series of products are as high as dozens of products, including health care products, food, Chinese patent medicines, etc. prestigious. But the host of Cordyceps sinensis is specific, the living environment is harsh, and artificial cultivation is difficult to be completely successful. Moreover, due to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01K67/033
CPCA01G18/00A01K67/033
Inventor 黄惠勇张水寒钟灿谢昭明朱如彩刘浩糜亚男王蕾
Owner 湖南省中医药研究院
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