Method for classification and identification of fish eggs and larval and juvenile fishes

A technology for larvae and juvenile fish and fish eggs, which is applied in the field of classification and identification of larvae and juvenile fish and fish eggs, can solve the problems of poor identification accuracy, large workload and long consumption time, and achieves small workload and high accuracy. , the effect of short consumption time

Inactive Publication Date: 2017-06-13
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

In the early resource investigation process, it is necessary to classify, identify and count the collected fish eggs, larvae and juveniles. The previous classification and identification required workers with certain basic knowledge and experience to conduct visual inspection and microscope observation, and the workload was heavy. It takes a long time, and the accuracy of some types of identification is not easy to grasp
When the number of fish eggs and juveniles surveyed and sampled is large, the previous classification and identification methods show obvious deficiencies

Method used

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Embodiment Construction

[0014] The present invention is a method for classifying and identifying fish roe and larvae, and adopts the following steps:

[0015] 1. Collect adult fish samples in the surveyed waters, classify and identify adult fish samples, take fresh muscle or fin ray tissue, fix with absolute ethanol, digest with proteinase K, extract DNA with phenol-imitation conventional methods, and store at -20°C spare;

[0016] 2. Select a relatively conservative COI sequence fragment, and use the general amplification primers: Forward primer: 5-TCAACCAACCACAAAGACATTGGCAC-3, Reverse primer:

[0017] 5-TAGACTTCTGGGTGGCCAAAGAATCA-3;

[0018] 3. The PCR amplification reaction system is: pre-denaturation at 95°C for 3 minutes; each cycle at 94°C for 30 seconds, 56°C for 30 seconds, and 72°C for 50 seconds, a total of 30 cycles; the last extension at 72°C for 6 minutes;

[0019] 4. After the PCR product was detected by 1% agarose gel electrophoresis, it was recovered with a purification kit and sequ...

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Abstract

The invention relates to a method for classification and identification of fish eggs and larval and juvenile fishes, and is characterized in that firstly, adult fish samples in an investigated water area are collected and are classified and identified, DNA is extracted from various classified and identified fish samples, COI sequences are amplified and sequenced by PCR respectively, and accordingly, a COI sequence database of the investigated water area fishes is established; then investigated and collected fish egg and larval and juvenile fish samples are numbered, and the COI sequences are amplified and sequenced by using the same method; the sequencing result is compared and analyzed with the established COI sequence database, and thus the types of the fish eggs and the larval and juvenile fishes are identified. The fish eggs and the larval and juvenile fishes at the fish early developmental stage are classified and identified fast, the shortages of difficult identification of fish eggs and larval and juvenile fishes, long time consuming and large workload are overcome, and fast, accurate and efficient advantages are showed.

Description

technical field [0001] The invention relates to the field of fishery, in particular to a method for classifying and identifying fish eggs and larvae. Background technique [0002] Early fish resource investigation is the investigation and research of fish resources in the early life history stage of fish, and it is an important means of fish ecology and fishery biology research. Early fish resource surveys can estimate the number of fish breeding populations, estimate the number of supplementary populations, scientifically predict changes in fish populations, and provide a scientific basis for the protection and rational development and utilization of fishery resources. In the early resource investigation process, it is necessary to classify, identify and count the collected fish eggs, larvae and juveniles. The previous classification and identification required workers with certain basic knowledge and experience to conduct visual inspection and microscope observation, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 朱书礼李新辉杨计平李捷李跃飞武智帅方敏
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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