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Method for research of feeding ecology of cephalopods in different life history stages according to eye crystalline lens

A technology of life history and cephalopods, applied in the field of aquaculture, can solve problems such as the inability to accurately analyze the feeding ecology of cephalopods, and achieve the effects of direct feeding ecology, easy extraction, and high accuracy

Inactive Publication Date: 2017-06-13
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, muscles can only analyze the feeding ecology of cephalopods in recent weeks. Although the inner shell and horny jaw can analyze the feeding ecology of the entire life history process, they cannot accurately analyze the cephalopods at different life history stages due to their own structural growth characteristics. Feeding ecology of pods

Method used

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  • Method for research of feeding ecology of cephalopods in different life history stages according to eye crystalline lens

Examples

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Effect test

Embodiment 1

[0049] Example 1: A method for studying the feeding ecology of cephalopods at different life history stages by using eye lenses

[0050] The operation steps are as follows:

[0051] 1. Extraction: cut the eyeball with a scalpel, and take out a pair of crystals with tweezers;

[0052] 2. Cleaning: Clean the crystal surface with clean water;

[0053] 3. Preservation: put the eye lens processed in step (2) into 75% alcohol for preservation;

[0054] 4. Separation: Take out the crystal, remove the distal end of the crystal, and keep the proximal end of the crystal;

[0055] 5. Soaking: soak the proximal crystal with distilled water for 5 minutes to make it fully absorb water;

[0056] 6. Measurement: Measure the diameter of the proximal crystal processed in step (5) with a vernier caliper;

[0057] 7. Micro-sampling: Under a dissecting microscope, use wide-head tweezers sterilized by absolute alcohol to peel off crystals with a width of about 0.5 mm from the outermost layer of...

Embodiment 2

[0068] Embodiment 2: the stripping method of eye lens section in different life history stages of cephalopods

[0069] The operation steps are as follows:

[0070] (1) Separation: take out the crystal, remove the distal end of the crystal, and keep the proximal end of the crystal; remove the smaller distal crystal while retaining the larger near-end crystal to ensure that there is enough crystal volume for micro-sampling. Complete stable isotope determination;

[0071] (2) Soaking: Soak the proximal crystal with distilled water for 5 minutes to make it fully absorb water, which will help the crystal to peel off;

[0072] (3) Measurement: measure the diameter of the near-end crystal that step (2) has processed with a vernier caliper;

[0073] (4) Micro-sampling: Under a dissecting microscope, use wide-head tweezers sterilized by absolute alcohol to peel off crystals with a width of about 0.5 mm from the outermost layer of the crystal to the core of the crystal; before micro-s...

Embodiment 3

[0080] Example 3: Method for Determination of Carbon and Nitrogen Stable Isotopes in Eye Lens Sections in Different Life History Stages of Cephalopods

[0081] The operation steps are as follows:

[0082] (1) Standard sample selection: select USGS 24 (-16.049‰V-PDB) and USGS 26 (53.7‰V-N2) standards as the calibration of stable isotopes of carbon and nitrogen;

[0083] (2) Determination of stable isotopes of carbon and nitrogen: Determination of stable isotopes of carbon and nitrogen was performed on samples of different cross-sections on an isotope ratio mass spectrometer.

[0084] Wherein, the determination step of stable isotope of carbon and nitrogen in the step (2) is:

[0085] (1) Drying: Put the crystal samples of different cross-sections into the drying oven for drying;

[0086] (2) Grinding: the dried sample is ground into powder in a frozen mixing ball mill, and sieved with a 100-mesh sieve;

[0087] (3) Weighing: Weigh about 1.5 mg of the sieved powder with a mil...

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Abstract

The invention relates to the field of aquatic products science, in particular to a method for research of feeding ecology of cephalopods in different life history stages according to eye crystalline lens. The method includes eye crystalline lens section stripping and stable carbon and nitrogen isotope determination in the different life history stages and mainly includes steps of extracting, cleaning, storing, crystalline lens layered stripping, drying, grinding, embedding, stable carbon and nitrogen isotope determination, feeding ecology analysis and the like. Different sections, representing different time periods, of eye near-end crystalline lens are subjected to layered stripping sampling from the periphery to the core, and an isotope ratio mass spectrometer is adopted for stable carbon and nitrogen isotope determination and feeding ecology analysis of eye crystalline lens different in section. By the method, the problem of difficulty in acquisition of feeding ecology information of the cephalopods in different life history stages is solved.

Description

technical field [0001] The invention relates to the field of fisheries, in particular to a method for studying the feeding ecology of cephalopods in different life history stages by using eye lenses. Background technique [0002] Cephalopods are the collective name for cephalopods of the mollusk phylum. They are important marine economic species and are widely distributed in the world's oceans and Antarctic sea areas. Cephalopods play a connecting role in the marine ecosystem, so their feeding ecology is the key research content of cephalopods, and has been widely concerned by scholars at home and abroad. Stomach content analysis is a traditional method to analyze the feeding ecology of cephalopods. In recent years, with the rise of nitrogen stable isotope analysis, tissues such as muscle, inner shell and horny jaw have been widely used to analyze the feeding ecology of cephalopods. However, muscles can only analyze the feeding ecology of cephalopods in recent weeks. Althou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 刘必林许巍陈新军高春霞林静远侯明磊李建华金岳蒋瑞倪震宇
Owner SHANGHAI OCEAN UNIV
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