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Temperature-sensitive glutamic acid fermentation production method with corn gluten meal hydrolysate replacing partial bean pulp hydrolysate

A technology of corn gluten powder and replacement parts, which is applied in fermentation and other directions, can solve the problems of high price and high cost of raw materials, and achieve the effect of simple operation, low cost and lightening burden

Inactive Publication Date: 2017-06-20
COFCO BIO CHEM ENERGY LONGJIANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soybean meal hydrolyzate is a commonly used organic nitrogen source in the thermosensitive glutamic acid fermentation production process, its price is relatively high, which makes the cost of raw materials high

Method used

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  • Temperature-sensitive glutamic acid fermentation production method with corn gluten meal hydrolysate replacing partial bean pulp hydrolysate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A temperature-sensitive glutamic acid fermentation production method that uses corn gluten powder hydrolyzate to replace part of soybean meal hydrolyzate. During the fermentation process, corn protein hydrolyzate is used to replace part of soybean meal hydrolyzate in the temperature-sensitive glutamic acid fermentation process to prepare fermentation culture Base, ferment and produce glutamic acid; the specific steps are as follows:

[0033] (1) The microbial strains used are thermosensitive glutamic acid bacteria.

[0034] (2) Fermentation medium: starch hydrolysis sugar 40g / L; corn gluten powder hydrolyzate 5g / L; soybean meal hydrolyzate 5g / L; molasses 5mL / L; disodium hydrogen phosphate 7g / L; potassium chloride 2g / L; Magnesium sulfate 1.4g / L; defoamer 0.1mL / L. The pH is 6.9-7.1, and it is sterilized at 121°C for 20 minutes.

[0035] (3) Additional sugar solution: the additional sugar solution is a glucose solution with a mass concentration of glucose of 600 g / L, and...

Embodiment 2

[0039] A temperature-sensitive glutamic acid fermentation production method that uses corn gluten powder hydrolyzate to replace part of soybean meal hydrolyzate. During the fermentation process, corn protein hydrolyzate is used to replace part of soybean meal hydrolyzate in the temperature-sensitive glutamic acid fermentation process to prepare fermentation culture Base, ferment and produce glutamic acid; the specific steps are as follows:

[0040] (1) The microbial strains used are thermosensitive glutamic acid bacteria.

[0041] (2) Fermentation medium: starch hydrolysis sugar 50g / L; corn gluten powder hydrolyzate 5g / L; soybean meal hydrolyzate 10g / L; molasses 10mL / L; disodium hydrogen phosphate 8g / L; potassium chloride 3g / L; Magnesium sulfate 1.5g / L; defoamer 0.2mL / L. pH is 6.9-7.1, sterilized at 121°C for 20 minutes;

[0042] The preparation method of described corn gluten powder hydrolyzate is as follows:

[0043] Corn gluten powder is hydrolyzed by acid method. The hy...

Embodiment 3

[0050] A temperature-sensitive glutamic acid fermentation production method that uses corn gluten powder hydrolyzate to replace part of soybean meal hydrolyzate. During the fermentation process, corn protein hydrolyzate is used to replace part of soybean meal hydrolyzate in the temperature-sensitive glutamic acid fermentation process to prepare fermentation culture Base, ferment and produce glutamic acid; the specific steps are as follows:

[0051] (1) The microbial strains used are thermosensitive glutamic acid bacteria.

[0052] (2) Fermentation medium: starch hydrolysis sugar 60g / L; corn gluten powder hydrolyzate 10g / L; soybean meal hydrolyzate 10g / L; molasses 15mL / L; disodium hydrogen phosphate 9g / L; potassium chloride 4g / L; Magnesium sulfate 1.6g / L; defoamer 0.3mL / L. pH is 6.9-7.1, sterilized at 121°C for 20 minutes;

[0053] The preparation method of described corn gluten powder hydrolyzate is as follows:

[0054] Corn gluten powder is hydrolyzed by acid method. The h...

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Abstract

The invention relates to a temperature-sensitive glutamic acid fermentation production method with corn gluten meal hydrolysate replacing partial bean pulp hydrolysate. In the fermentation process, the bean pulp hydrolysate in a temperature-sensitive glutamic acid fermentation process is replaced by using corn gluten meal hydrolysate, and thus glutamic acid can be produced through fermentation. The invention further provides a method for preparing a temperature-sensitive glutamic acid fermentation culture medium from corn gluten protein powder as a raw material which is hydrolyzed and replaces a part of the bean pulp hydrolysate. Production indexes are generally accordant with those of normal fermentation, and the raw material is a byproduct of starch production, so that the cost is low; while stable production is ensured, the comprehensive production cost of the glutamic acid can be effectively reduced through fermentation production, the economic benefits of enterprises can be directly increased, and thus the method is applicable to comprehensive popularization in production.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, in particular to a thermosensitive glutamic acid fermentation production method which uses corn gluten powder hydrolyzate to replace part of soybean meal hydrolyzate. Background technique [0002] Thermosensitive glutamic acid fermentation raw materials need rich organic nitrogen sources to provide various amino acids and organic nitrogen necessary for the growth and reproduction of bacteria and metabolic acid production, so that the bacteria grow robustly, enhance their vitality, and accumulate a large amount of glutamic acid. Soybean meal hydrolyzate is a commonly used organic nitrogen source in the thermosensitive glutamic acid fermentation production process, and its price is relatively high, which makes the cost of raw materials high. [0003] Corn gluten meal is a by-product of the production of starch from corn grains. It has a high protein content and also cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14
CPCC12P13/14
Inventor 佟毅王晓健韩隽姬慧军李大勇王兰刚丛泽峰宋辉王淑玲张清稳
Owner COFCO BIO CHEM ENERGY LONGJIANG CO LTD