Application of n-3PUFA phosphatide in products for alleviating chronic fatigue syndromes

A technology for chronic fatigue and syndrome, applied in the directions of edible phospholipid composition, application, medical preparations containing active ingredients, etc., can solve problems such as difficulty in digestion and absorption, potential safety hazards, etc., to prolong running time and improve fatigue resistance. Receptive, the best effect

Inactive Publication Date: 2017-06-23
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] DHA and EPA are two common n-3 series unsaturated fatty acids. The EPA / DHA products currently on the market are mainly ethyl ester type and glyceride type, and the ethyl ester type EPA / DHA is not only difficult to digest and absorb in the human body , and there may be potential safety hazards, so glyceride EPA / DHA is very popular among consumers

Method used

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  • Application of n-3PUFA phosphatide in products for alleviating chronic fatigue syndromes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of sea cucumber EPA phospholipid

[0030] (1) Commercially available North Atlantic melon ginseng was vacuum freeze-dried and pulverized, then ground into powder (200 mesh).

[0031] In the second step, take 10kg of sea cucumber dry powder, add 100L chloroform / methanol (2:1, v / v), stir, filter, extract with 0.88% potassium chloride solution, let stand for layers, collect the chloroform layer, and extract 3 times, the concentrated solution was combined and concentrated under reduced pressure to obtain the crude extract of sea cucumber or squid roe lipid;

[0032] The third step is to weigh 200g of silica gel, add 5% water, activate at 120°C for 4h, and cool at room temperature. The column was packed by wet method, and after the silica gel settled completely, the column was pressed with 2 times the column volume of chloroform to make the silica gel in the column more dense. After the sea cucumber or squid roe crude extract sample of 50g was...

Embodiment 2

[0036] Embodiment 2: the preparation of squid egg DHA phospholipid

[0037] (1) Commercially available cuttlefish eggs were vacuum freeze-dried and pulverized, then ground into powder (200 mesh).

[0038]In the second step, take 10kg of squid roe dry powder, add 100L chloroform / methanol (2:1, v / v), stir, filter, extract with 0.88% potassium chloride solution, let stand to separate layers, collect the chloroform layer, soak Extract 3 times, combine the concentrated solution, and concentrate under reduced pressure to obtain the crude extract of squid roe lipid;

[0039] The third step is to weigh 200g of silica gel, add 5% water, activate at 120°C for 4h, and cool at room temperature. The column was packed by wet method, and after the silica gel settled completely, the column was pressed with 2 times the column volume of chloroform to make the silica gel in the column more dense. After the squid roe crude extract sample of 50g is dissolved with as little chloroform as possible...

Embodiment 3

[0043] Embodiment 3: animal experiments

[0044] 1. Materials and Methods

[0045] (1) Animal feeding and behavioral analysis

[0046] As experimental animals, Balb / c mice, male, weighing 22±0.5g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. The humidity in the animal room was 65±15%, the room temperature was 23±2°C, light and dark alternated at 12:12 h, and the mice were free to eat and drink. After one week of adaptive feeding, the mice were divided into 10 groups according to body weight: normal control group, model control group, DHA-PC, DHA-PE, DHA-PS, DHA-PI and EPA-PC, EPA-PE , EPA-PS, EPA-PI groups, 8 rats in each group, were fed with corresponding feeds. The feed was modified with reference to the AIN-93G rodent feed formula. The experimental group was added with 0.5% DHA+EPA. The food intake was recorded and counted every day, and the weight change was recorded every other day. The feeding was continued for 3 weeks. Every oth...

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Abstract

The invention provides an application of n-3PUFA phosphatide in products for alleviating chronic fatigue syndromes. Specifically, EPA phosphatide is extracted from trepang, DHA phosphatide is extracted from squid eggs, components of the EPA phosphatide are prepared, components of the DHA phosphatide are separated, and finally weariness resistance test verification is performed on the components of the EPA phosphatide and the components of the DHA phosphatide. According to the application disclosed by the invention, the EPA phosphatide is prepared by using the trepang as a raw material, the DHA phosphatide is prepared by using the squid eggs as a raw material, then component separation and transformation are performed, and the purity of prepared PC, the purity of the prepared PE, the purity of the prepared PS and the purity of the prepared PI are all 90% or above. According to the application disclosed by the invention, animal experiments verify that the components of the prepared EPA phosphatide and the components of the prepared DHA phosphatide can greatly prolong the running time of mice in a fatigue apparatus, namely that the EPA phosphatide and the DHA phosphatide can both obviously improve the fatigue tolerance of mice suffering from chronic fatigue syndromes under moderate intensity, and the EPA phosphatide and the DHA phosphatide can effectively prevent, alleviate or treat the chronic fatigue syndromes.

Description

technical field [0001] The invention belongs to the technical field of active substance screening, and relates to the application of n-3PUFA phospholipids in improving chronic fatigue syndrome products, in particular to the application of sea cucumber phospholipids and squid lecithin in preventing, improving or treating chronic fatigue syndrome disorders. Background technique [0002] Chronic fatigue syndrome (chronic fatigue syndrome, CFS), also known as chronic fatigue immune dysfunction syndrome, is a special manifestation of sub-health state. A group characterized by long-term severe fatigue (at least half a year) as the prominent clinical manifestation, accompanied by insomnia, memory loss, skeletal muscle pain and various psychoneurological symptoms, but without other organic and mental diseases Complex dysfunction syndrome. Domestic surveys show that the incidence of CFS population is about 0.007% to 2.8%, and most of the patients are between 30 and 50 years old. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/115A61K31/685C07F9/10A61P39/00
CPCA61K31/685C07F9/103
Inventor 王玉明王丹徐杰薛勇薛长湖李兆杰王静凤常耀光唐庆娟
Owner OCEAN UNIV OF CHINA
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