Epothilone B purification method

Abstract

The invention relates to an improved method for epothilone B extraction and purification, and belongs to the technical field of biological separation. According to the method, most of impurities are removed by a low-temperature refrigeration method, solid-liquid separation is achieved by changing density, the purity of finished products is further improved by a two-time crystal method, rate-limiting steps such as silica-gel column chromatography are decreased, an epothilone B extraction process is simplified, operation cost is reduced, and the method has a wider application prospect.

Description

technical field

[0001] The invention belongs to the technical field of biological separation, and in particular relates to a method for extracting and purifying epothilone B. Background technique

[0002] The discovery of paclitaxel has pushed the research of anticancer drugs into a new field, but with the in-depth research and clinical application, paclitaxel has exposed many shortcomings and drawbacks, including the difficulty of synthesis, the lack of extraction resources and the lack of yew trees in clinical applications. side effect. Later, in 1985, the GBF Institute in Germany isolated a myxobacteria, Somatocystis cellulosus Soce90 strain, from the soil of the Zambezi River beach in South Africa in 1985, and measured its culture to have antifungal activity and cytotoxicity. Around 1995, Daniel M.Bollag of the West Point Merck Research Laboratory in Pennsylvania, USA, etc., in order to seek compounds with tubulin (Tubulin) polymerization activity, obtained from the J.E...